Diagnostic test for CSFV antibodies

Patent metadata

Field	Value
Publication number	US-9739778-B2
Application number	US-201415104406-A
Country	US
Kind code	B2
Filing date	Dec 18, 2014
Priority date	Dec 19, 2013
Publication date	Aug 22, 2017
Grant date	Aug 22, 2017

Abstract

The present invention relates to the field of veterinary diagnostics, specifically to a test for the detection of antibodies against CSFV. In particular the invention relates to a method for detecting antibodies against wild type CSFV in a test sample, characterized in that the method comprises co-incubating with a carrier comprising a mutated TAVSPTTLR epitope of CSFV E2 protein. Further, the invention relates to a diagnostic test kit, and to the use of the method according to the invention. In addition the invention relates to a method for differentiating between animals infected with wild type CSFV and animals that were vaccinated against CSFV with a CSFV (marker) vaccine, and to a method for controlling an infection with wild type CSFV in a population of porcine animals, by the combined use of a CSFV (marker) vaccine and the diagnostic test kit of the invention.

First claim

The invention claimed is:

1. A method for detecting antibodies against wildtype classical swine fever virus (CSFV) in a test sample, whereby said sample may also comprise antibodies against a mutated TAVSPTTLR epitope of CSFV E2, the method comprising a step for incubating said test sample with an immobilized carrier comprising a TAVSPTTLR epitope of CSFV E2 comprising the amino acid sequence of SEQ ID NO: 1; wherein the method comprises co-incubating in said step with a carrier comprising a mutated TAVSPTTLR epitope of CSFV E2 comprising the amino acid sequence selected from the group consisting of SEQ ID NO: 2 and SEQ ID NO: 3.

2. The method of claim 1 , wherein the detection is performed by ELISA (enzyme linked immunosorbant assay).

3. The method of claim 1 , wherein the test sample is obtained from a porcine animal that had been vaccinated against CSF with a CSFV vaccine comprising a CSFV E2 protein comprising the mutated TAVSPTTLR epitope.

4. A diagnostic test kit for implementing the method of claim 3 .

5. The method of claim 1 , wherein the mutated TAVSPTTLR epitope of CSFV E2 that is comprised in the carrier for use in co-incubating, is the same as the mutated TAVSPTTLR epitope comprised in the CSFV E2 protein of a CSFV vaccine that was used to vaccinate the porcine animals from which the test sample was obtained.

6. The method of claim 5 , wherein the CSFV vaccine was based on the vFlc-ΔPTa1 virus.

7. A diagnostic test kit for implementing the method of claim 5 .

8. The method of claim 1 , wherein the mutated TAVSPTTLR epitope of CSFV E2 that is comprised in the carrier for use in co-incubating comprising the amino acid sequence of TAGSTLRTE (SEQ ID NO: 2).

9. The method of claim 8 , wherein the CSFV vaccine was based on a vFlc-ΔPTa1 virus.

10. A diagnostic test kit for implementing the method of claim 8 .

11. The method of claim 1 , wherein either the immobilised carrier comprising a TAVSPTTLR epitope of CSFV E2, or the carrier comprising a mutated TAVSPTTLR epitope of CSFV E2, is a CSFV E2 protein, or wherein both the carriers are CSFV E2 proteins.

12. The method of claim 1 , comprising adapting the incubation conditions of the step comprising co-incubating with a carrier comprising the mutated TAVSPTTLR epitope of CSFV E2, to accommodate for the addition of said carrier.

13. A diagnostic test kit for implementing the method of claim 1 .

14. A method for controlling an infection with wildtype CSFV in a population of porcine animals, by the combined use of a CSFV vaccine comprising a CSFV E2 protein comprising a mutated TAVSPTTLR epitope, and the diagnostic test kit of claim 13 .

Assignees

• Intervet Inc

Inventors

• Bruderer Urs Peter

Classifications

• A61K39/12

  Viral antigens · CPC title

• G01N2469/20

  Detection of antibodies in sample from host which are directed against antigens from microorganisms · CPC title

• C12N2800/22

  Vectors comprising a coding region that has been codon optimised for expression in a respective host · CPC title

• C12N2770/24322

  New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes · CPC title

• G01N2333/183

  Flaviviridae, e.g. pestivirus, mucosal disease virus, bovine viral diarrhoea virus, classical swine fever virus (hog cholera virus) or border disease virus · CPC title