Insect resistant and herbicide tolerant breeding stack of soybean event pDAB9582.814.19.1 and pDAB4468.04.16.1

Patent metadata

Field	Value
Publication number	US-9738904-B2
Application number	US-201214234923-A
Country	US
Kind code	B2
Filing date	Jul 26, 2012
Priority date	Jul 26, 2011
Publication date	Aug 22, 2017
Grant date	Aug 22, 2017

Abstract

A breeding stack of soybean event 9582.814.19.1 and soybean event pDAB4468.04.16.1 resulted in the novel soybean event pDAB9582.814.19.1::pDAB4468.04.16.1. Soybean event pDAB9582.814.19.1::pDAB4468.04.16.1 comprises genes encoding AAD-12, Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products.

First claim

The invention claimed is:

1. A method of controlling insects, said method comprising contacting insects with insect resistant soybean plants of event pDAB9582.814.19.1::pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 14, encoding cry1F, cry1Ac and phosphinothricin acetyltransferase (pat), and SEQ ID NO: 15, encoding aryloxyalkanoate dioxygenase-12 (aad-12) and pat, to thereby control insects selected from the group consisting of Pseudoplusia includens, Anticarsia gemmatalis and Spodoptera frugiperda.

2. The method of claim 1 wherein said insects are Pseudoplusia includens.

3. The method of claim 1 wherein said insects are Anticarsia gemmatalis.

4. The method of claim 1 wherein said insects are Spodoptera frugiperda.

5. A method of controlling weeds in an area comprising a soybean crop, said method comprising applying a glufosinate herbicide, a phenoxyacetic acid herbicide, a pyridyloxyacetic acid herbicide, or combinations thereof to said, wherein soybean plants of said soybean crop comprise event pDAB9582.814.19.1::pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 14, encoding cry1F, cry1Ac and pat, and SEQ ID NO: 15, encoding aad-12 and pat.

6. An isolated DNA molecule comprising one and more sequences selected from the group consisting of nucleotides 1385-1415 of SEQ ID NO: 1; nucleotides 1350-1450 of SEQ ID NO: 1; nucleotides 1300-1500 of SEQ ID NO: 1; nucleotides 1200-1600 of SEQ ID NO: 1; nucleotides 137-168 of SEQ ID NO:2; bp 103-203 of SEQ ID NO:2; and bp 3-303 of SEQ ID NO:2; or one or more sequences selected from the group consisting of nucleotides 2680-2780 of SEQ ID NO: 15; nucleotides 2630-2830 of SEQ ID NO:15; nucleotides 2530-2930 of SEQ ID NO:15; nucleotides 9071-9171 of SEQ ID NO:15; nucleotides 9021-9221 of SEQ ID NO: 15; and nucleotides 8921-9321 of SEQ ID NO: 15.

7. A method of breeding a soybean plant, comprising:
crossing a first soybean plant with a second soybean plant to produce a third soybean plant, wherein said first plant is a plant of event pDAB9582.814.19.1, wherein said event comprises SEQ ID NO: 14, encoding cry1F, cry1Ac and pat, and wherein said second plant is a plant of event pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 15, encoding aad-12 and pat; and
assaying said third soybean plant for presence of DNA comprising said SEQ ID NO: 14 and SEQ ID NO: 15.

8. An isolated DNA molecule comprising a junction sequence comprising at least one sequence selected from the group consisting of nucleotides 1385-1415 of SEQ ID NO:1; nucleotides 1350-1450 of SEQ ID NO:1; nucleotides 1300-1500 of SEQ ID NO:1; nucleotides 1200-1600 of SEQ ID NO:1; nucleotides 137-168 of SEQ ID NO:2; nucleotides 103-203 of SEQ ID NO:2; nucleotides 3-303 of SEQ ID NO:2 and full complements thereof; and comprising at least one sequence selected from the group consisting of bp 2680-2780 of SEQ ID NO:15; nucleotides 2630-2830 of SEQ ID NO: 15; nucleotides 2530-2930 of SEQ ID NO:15; nucleotides 9071-9171 of SEQ ID NO:15; nucleotides 9021-9221 of SEQ ID NO:15; nucleotides 8921-9321 of SEQ ID NO:15 and full complements thereof.

9. A soybean plant that is resistant to Pseudoplusia includens , wherein said plant comprises event pDAB9582.814.19.1::pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 14, encoding cry1F, cry1Ac and pat, and SEQ ID NO: 15, encoding aad-12 and pat.

10. A seed of the plant of claim 9 , wherein said seed comprises said event.

11. Soybean meal produced from the plant of claim 9 , wherein said meal comprises said event.

12. A method of controlling insect pests selected from the group consisting of Pseudoplusia includens, Anticarsia gemmatalis , and Spodoptera frugiperda , in soybean seed, meal, or flour, said method comprising contacting said insects with seed, meal, or flour that comprises event pDAB9582.814.19.1::pDAB4468.04.16.1, wherein said event comprises SEQ ID NO: 14 encoding cry1F, cry1Ac and pat, and SEQ ID NO: 15, encoding aad-12 and pat.

13. A part of the soybean plant of claim 9 , wherein said part is selected from the group consisting of pollen, ovule, flowers, shoots, roots, and leaves, wherein said part comprises said event.

Assignees

• Cui Yunxing C
• Hoffman Thomas
• Parkhurst Dawn M
• Zhou Ning
• Wiggins Barry
• Pareddy Dayakar
• Bradfisch Gregory A
• Dripps James E
• Toledo Sandra G
• Bard Nathan
• Vercauteren Michael
• Nagaraj Nandi
• Bishop Brandon
• Gilles Gregory J
• Wright Terry R
• Colon Julissa
• Barnes Ricardo A
• Vanopdorp Nathan J
• Bai Yonghe
• Dow Agrosciences Llc

Inventors

• Cui Yunxing C
• Hoffman Thomas
• Parkhurst Dawn M
• Zhou Ning
• Wiggins Barry
• Pareddy Dayakar
• Bradfisch Gregory A
• Dripps James E
• Toledo Sandra G
• Bard Nathan
• Vercauteren Michael
• Nagaraj Nandi
• Bishop Brandon
• Gilles Gregory J
• Wright Terry R
• Colon Julissa
• Barnes Ricardo A
• Vanopdorp Nathan J
• Bai Yonghe

Classifications

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Q2600/13

  Plant traits · CPC title

• A01N37/44

  containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids · CPC title

• C12N5/10

  Cells modified by introduction of foreign genetic material · CPC title

• A01H5/00 Primary

  Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy · CPC title