Affinity reagents for protein purification

Patent metadata

Field	Value
Publication number	US-9737875-B2
Application number	US-201314385586-A
Country	US
Kind code	B2
Filing date	Mar 15, 2013
Priority date	Mar 16, 2012
Publication date	Aug 22, 2017
Grant date	Aug 22, 2017

Abstract

Disclosed herein are methods and compositions for purifying proteins from crude solutions.

First claim

What is claimed is:

1. A compound comprising the structure

wherein the wavy line indicates the point of attachment to a substrate, optionally through a linker;
wherein R 1 is selected from the group consisting of 2-phenylpropylamine, 4-methylbenzylamine, 2-phenylethanamine, benzylamine, 3-phenylpropylamine, 4-(2-aminomethyl)benzenesulfonamide, 2-methylbutylamine, isoamylamine, isobutylamine, butylamine, propargylamine, piperazine, ethylamine, 1-amino-3,3-diethoxypropane, 4-amino-2-methyl-1-butanol, 5-amino-1-pentanol, acetamide, 2-amino-N-cyclopropyl-1-amino-2-butanol, N,N-bis(2-aminoethyl)ethane-1,2-diamine, N,N,2,2-tetramethyl-1,3-propanediamine, N,N-diethyl-1,3-propanediamine, N1-(2-aminoethyl)-N1-methylethane-1,2-diamine, N,N-dimethyl-1,3-propanediamine, 4-(difluoromethoxy)benzylamine, 2-N-propoxyethylamine, 2-methoxyethylamine, N-tert-butoxycarbonyl-3-aminopropanamine, 3-isopropoxypropylamine, 3-(methylthio)propylamine, 2-aminoethyl isopropyl ether, 4-bromo-2-fluorobenzylamine, 4-iodobenzylamine, 4-bromobenzylamine, 2-(4-nitro-phenyl)-ethylamine, 3-fluoro-5-(trifluoromethyl)benzylamine, 4-nitro-benzylamine, 3-bromobenzylamine, 3-chloro-2,6-difluorobenzylamine, 3,4-dichlorobenzylamine, 4-(trifluoromethyl)benzylamine, 2-(3-chlorophenyl)ethylamine, 2-(4-chlorophenyl)ethylamine, 3-chlorobenzylamine, 2-chlorobenzylamine, 3-fluorophenethylamine, 4-fluorophenethylamine, 3-fluorobenzylamine, tetrahydrofurfurylamine, 4-(2-aminoethyl)-1-benzylpiperidine, (2,2-dimethyl-[1,3]-dioxolan-4-yl)-methylamine, N-(3-aminopropyl)morpholine, 4-(2-aminoethyl)morpholine, 2-(2-aminoethyl)-1-methylpyrrolidine, 4-(aminomethyl)piperidine, 2-(aminomethyl)-1-ethylpyrrolidine, 4-pyrrolidinobutylamine, 1-(3-aminopropyl)pyrrolidine, 1-(3-aminopropyl)-4-methylpiperazine, N-(3′-aminopropyl)-2-pyrrolidinone, ethyl 4-amino-1-piperidinecarboxylate, ethanone, 2-amino-1-(4-morpholinyl)-3-aminopropane-1,2-diol, 3-aminopropionitrile, 3-amino-1-propanol, 2-(2-aminoethoxy)ethanol, 2-(1h-indol-3-yl)ethanamine (tryptamine), 3-(2-aminoethyl)pyridine, 1h-indole-2-methanamine, acetamide, 2-amino-N-(1-methylethyl)-, 2-(2-methoxyphenyl)ethylamine, 2-(aminomethyl)pyridine, 4-(2-aminoethyl)pyridine, 2-thiophenemethylamine, 4-(aminomethyl)pyridine, 3-(aminomethyl)pyridine, 3-bromo-4-methoxyphenethylamine, 3-furanmethanamine, 2-quinolinemethanamine, homopiperazine, propanamide, 3-amino-N-methyl-, 2-(2-furylmethylthio)ethanamine, 4-(2-aminoethyl)benzenesulfonamide, 5-aminomethyl-2-chloropyridine, 5-methoxytryptamine, N-acetylethylenediamine, s-(2-aminoethyl)isothiouronium bromide, homotaurine, (r)-1-(3-methoxyphenyl)ethylamine, 2-(3,4-dimethoxyphenyl)ethylamine, 3-methoxyphenethylamine, 2-(4-methoxyphenyl)ethylamine, 4-methoxybenzylamine, 2-phenoxyethylamine, 2-methoxybenzylamine, and 3-methoxybenzylamine;

R 2 is selected from the group consisting of 2-phenylpropylamine, 4-methylbenzylamine, 2-phenylethanamine, benzylamine, 3-phenylpropylamine, 4-(2-aminomethyl)benzenesulfonamide, 2-methylbutylamine, isoamylamine, isobutylamine, butylamine, propargylamine, piperazine, ethylamine, 1-amino-3,3-diethoxypropane, 4-amino-2-methyl-1-butanol, 5-amino-1-pentanol, acetamide, 2-amino-N-cyclopropyl-, 1-amino-2-butanol, N,N-bis(2-aminoethyl)ethane-1,2-diamine, n,n,2,2-tetramethyl-1,3-propanediamine, N,N-diethyl-1,3-propanediamine, n1-(2-aminoethyl)-n1-methylethane-1,2-diamine, N,N-dimethyl-1,3-propanediamine, 4-(difluoromethoxy)benzylamine, 2-N-propoxyethylamine, 2-methoxyethylamine, N-tert-butoxycarbonyl-3-aminopropanamine, 3-isopropoxypropylamine, 3-(methylthio)propylamine, 2-aminoethyl isopropyl ether, 4-bromo-2-fluorobenzylamine, 4-iodobenzylamine, 4-bromobenzylamine, 2-(4-nitro-phenyl)-ethylamine, 3-fluoro-5-(trifluoromethyl)benzylamine, 4-nitro-benzylamine, 3-bromobenzylamine, 3-chloro-2,6-difluorobenzylamine, 3,4-dichlorobenzylamine, 4-(trifluoromethyl)benzylamine, 2-(3-chlorophenyl)ethylamine, 2-(4-chlorophenyl)ethylamine, 3-chlorobenzylamine, 2-chlorobenzylamine, 3-fluorophenethylamine, 4-fluorophenethylamine, 3-fluorobenzylamine, tetrahydrofurfurylamine, 4-(2-aminoethyl)-1-benzylpiperidine, (2,2-dimethyl-[1,3]-dioxolan-4-yl)-methylamine, N-(3-aminopropyl)morpholine, 4-(2-aminoethyl)morpholine, 2-(2-aminoethyl)-1-methylpyrrolidine, 4-(aminomethyl)piperidine, 2-(aminomethyl)-1-ethylpyrrolidine, 4-pyrrolidinobutylamine, 1-(3-aminopropyl)pyrrolidine, 1-(3-aminopropyl)-4-methylpiperazine, N-(3′-aminopropyl)-2-pyrrolidinone, ethyl 4-amino-1-piperidinecarboxylate, ethanone, 2-amino-1-(4-morpholinyl)-, 3-aminopropane-1,2-diol, 3-aminopropionitrile, 3-amino-1-propanol, 2-(2-aminoethoxy)ethanol, 2-(1H-indol-3-yl)ethanamine (tryptamine), 3-(2-aminoethyl)pyridine, 1H-indole-2-methanamine, acetamide, 2-amino-N-(1-methylethyl)-, 2-(2-methoxyphenyl)ethylamine, 2-(aminomethyl)pyridine, 4-(2-aminoethyl)pyridine, 2-thiophenemethylamine, 4-(aminomethyl)pyridine, 3-(aminomethyl)pyridine, 3-bromo-4-methoxyphenethylamine, 3-furanmethanamine, 2-quinolinemethanamine, homopiperazine, propanamide, 3-amino-N-methyl-, 2-(2-furylmethylthio)ethanamine, 4-(2-aminoethyl)benzenesulfonamide, 5-aminomethyl-2-chloropyridine, 5-methoxytryptamine, N-acetylethylenediamine, s-(2-aminoethyl)isothiouronium bromide, homotaurine, (r)-1-(3-methoxyphenyl)ethylamine, 2-(3,4-dimethoxyphenyl)ethylamine, 3-methoxyphenethylamine, 2-(4-methoxyphenyl)ethylamine, 4-methoxybenzylamine, 2-phenoxyethylamine, 2-methoxybenzylamine, 3-methoxybenzylamine, (z)-4-chloro-2-buten-1-amine, 2-(1-cyclohexenyl)ethylamine, 3-methoxypropylamine, 3-ethoxypropylamine, 3-N-propoxypropylamine, 2-ethoxyethylamine, 3-iodobenzylamine, 3-(trifluoromethyl)benzylamine, 4-(4-fluorophenoxy)benzylamine, 3,4-methylenedioxybenzylamine, 8-quinolinemethanamine, 4-pyridinemethanamine, 2-(dimethylamino)-, 2-aminoethyl hydrogen sulfate, aminomethanesulfonic acid, 3,4,5-trimethoxybenzylamine, dl-1-(1-naphthyl)ethylamine, (s)-(−)-1-(4-methylphenyl)ethylamine, (s)-(−)-beta-methylphenethylamine, (r)-(−)-1-aminoindan, (s)-(−)-1-phenylethylamine, (r)-(+)-1-phenylethylamine, 1-phenylbutan-3-amine, d-phenylalaninol, (1r,2s)-1-amino-2-indanol, 2-amino-3,3-dimethylbutane, 1,3-dimethylbutylamine, sec-butylamine, isopropylamine, 2-amino-1-methoxybutane, (s)-2-amino-1-phenylethanol, (s)-(+)-2-amino-1-pentanol, (r)-(−)-2-amino-1-butanol, 1-leucinamide, cyclopentylamine, cyclopropylamine, trans-4-aminocyclohexanol, (1s,2s)-(+)-2-benzyloxycyclohexylamine, (1r,2r)-(−)-2-benzyloxycyclopentylamine, 2-(2-chlorophenyl)ethylamine, 2-fluorophenethylamine, (1r,2r)-(−)-2-amino-1-(4-nitrophenyl)-1,3-propanediol, 4-aminotetrahydropyran, dl-homocysteine thiolactone, dl-α-amino-ε-caprolactam, indan-5-ylamine, 4-aminopyridine, N-(3-aminopropyl)-N-methylaniline, 2-fluoroethanamine, alpha-phenylglycinonitrile, (1r,2r)-(−)-2-amino-1-phenyl-1,3-propanediol, beta alanine napthylamide, 1-leucine-4-nitroanilide, 2-amino-3-methyl-3-sulfanylbutanoic acid, (s)-(−)-1-(3-methoxyphenyl)ethylamine, (s)-(−)-1-(4-methoxyphenyl)ethylamine, trans-2,5-dimethylpiperazine, 9-aminofluorene, 2,4,6-trimethylaniline, 2,4-dimethylaniline, 3,5-dimethyl aniline, 2,5-dimethylaniline, 2-methylaniline, 2,6-diethylaniline, 3-isopropylaniline, 4-isopropylaniline, m-aminobenzoylamine, 4-aminobenzamide, 3-nitroaniline, 2,6-difluoroaniline, 3′,4′-difluoroaniline, 3-chloroaniline, 2-chloroaniline, 2-fluoro-aniline, 4-fluoroaniline, 3,4-methylenedioxy-1-aminobenzene, 2-aminopyrazine, 3-aminopyridine, 2-aminopyridine, 4-methyl-1,3-thiazol-2-amine, 2-aminopyrimidine, 5-amino-1h-tetrazole, 4-ethoxyaniline, 2-(methylmercapto)aniline, 2-ethoxyaniline, o-anisidine, and 4-amino-1-butanol;
R 3 is selected from the group consisting of 2-phenylpropylamine, 4-methylbenzylamine, 2-phenylethanamine, benzylamine, 3-phenylpropylamine, 4-(2-aminomethyl)benzenesulfonamide, 2-methylbutylamine, isoamylamine, isobutylamine, butylami

Assignees

• Impossible Foods Inc
• Univ Leland Stanford Junior

Inventors

• Brown Patrick O'Reilly
• Watts R Edward
• Harbury Pehr

Classifications

• G01N33/543

  with an insoluble carrier for immobilising immunochemicals · CPC title

• B01J20/3212

  consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds · CPC title

• C07D251/70

  Other substituted melamines · CPC title

• A61K31/53

  having six-membered rings with three nitrogens as the only ring hetero atoms, e.g. chlorazanil, melamine (melarsoprol A61K31/555 {; with four nitrogen atoms A61K31/495}) · CPC title

• C07K1/22 Primary

  Affinity chromatography or related techniques based upon selective absorption processes · CPC title