Systems And Methods Of Reduced Condensation Microscopy
US-2024345386-A1 · Oct 17, 2024 · US
US9733465B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9733465-B2 |
| Application number | US-201615041384-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 11, 2016 |
| Priority date | Feb 12, 2015 |
| Publication date | Aug 15, 2017 |
| Grant date | Aug 15, 2017 |
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Evanescently-coupled planar waveguides for enhancing total internal reflection fluorescence microscopy are disclosed. The waveguides include multiple thin layers of one or more materials on a cover slip arranged resonantly enhance the optical field at the surface of the layer stack by evanescently coupling to a leaky guided mode.
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What is claimed is: 1. A cover slip for resonant enhancement of an evanescent optical field, comprising a cover slip having: (i) a coupling layer directly adjacent a glass substrate having a refractive index n c , and a thickness more than about one-half an optical excitation wavelength; and (ii) one or more guide layers on the coupling layer having a refractive index n gi that is more than a medium of a specimen; wherein n c is less than refractive indices of any layer subseque…
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