Nuclear magnetic resonance (NMR) spectroscopy device

Patent metadata

Field	Value
Publication number	US-9733323-B2
Application number	US-201214367547-A
Country	US
Kind code	B2
Filing date	Dec 21, 2012
Priority date	Dec 22, 2011
Publication date	Aug 15, 2017
Grant date	Aug 15, 2017

Abstract

The invention relates to a Nuclear Magnetic Resonance (NMR) spectroscopy device adapted for carrying out 1D and nD homo- and heteronuclear NMR spectroscopy measurements of a plurality of nuclei, comprising an RF coil adapted to transmit RF to and/or receive RF from a measuring volume, wherein the RF coil forms part of a non-tuned radiofrequency circuit. The invention further relates to a method of NMR data acquisition, a method of manufacturing a NMR spectroscopy device and a NMR-device holder.

First claim

The invention claimed is:

1. A Nuclear Magnetic Resonance (NMR) spectroscopy device for carrying out one dimensional and/or multidimensional heteronuclear spectroscopy measurements of a plurality of nuclides, the NMR spectroscopy device comprising:
a magnet for maintaining a substantially constant magnetic field in a measuring volume; and
a fluidic device, including a chip substrate, wherein a micro-coil is provided on the chip substrate, the micro-coil being an RF coil adapted to transmit RF radiation to and/or receive RF radiation from the measuring volume,
wherein the micro-coil connects to and forms part of a non-tuned non-matched radiofrequency circuit.

2. The NMR spectroscopy device according to claim 1 , wherein the RF coil has a detection volume of less than 1 milliliter.

3. The NMR spectroscopy device of claim 2 , wherein the detection volume is less than 100 microliters.

4. The NMR spectroscopy device of claim 3 , wherein the detection volume is less than 100 nanoliters.

5. A method of carrying out Nuclear Magnetic Resonance (NMR) one dimensional and/or multidimensional heteronuclear spectroscopy measurements of a plurality of nuclides, the method comprising the steps of:
providing a sample comprising a plurality of nuclides in the measuring volume of the NMR spectroscopy device of claim 1 :
executing a data acquisition sequence on the NMR spectroscopy device;
acquiring data from the sample pursuant to said data acquisition sequence.

6. The method according to claim 5 , wherein the data acquisition sequence includes decoupling on one of the nuclides' Larmor frequencies.

7. The NMR spectroscopy device of claim 1 , further comprising holder configured for allowing the hosting of the fluidics device, said holder comprising a first side and a second side, wherein
the first side is provided with a non-tuned RF circuit having electrodes for connecting to the fluidic device;
and the second side is provided with fluidic connection points,
wherein the holder is manufactured from a non-magnetic material.

8. The NMR-device holder according to claim 7 , comprising first holes and second holes, the first holes correspond to the electrodes on the first side and the second holes correspond to fluidic connection points on the second side.

9. The NMR spectroscopy device of claim 7 , wherein the holder is manufactured from aluminum.

10. The NMR spectroscopy device of claim 1 , wherein the micro-coil is a spiral coil.

11. The NMR spectroscopy device of claim 1 , further comprising a sample inlet/outlet provided on the chip substrate.

12. The NMR spectroscopy device of claim 11 , wherein a fluidic channel, configured to dwell in the measuring volume, is centered on the chip substrate.

13. The NMR spectroscopy device of claim 11 , wherein the micro-coil is positioned on a bottom of the chip substrate.

Assignees

• Univ Wageningen

Inventors

• Velders Aldo Hendrikus
• Fratila Raluca Maria
• Gomez Almagro Maria Victoria
• Sykora Stanislav

Classifications

• G01R33/465

  applied to biological material, e.g. in vitro testing · CPC title

• G01R33/46

  NMR spectroscopy · CPC title

• G01R33/30

  Sample handling arrangements, e.g. sample cells, spinning mechanisms · CPC title

• G01R33/3635

  Multi-frequency operation · CPC title

• G01R33/302 Primary

  Miniaturized sample handling arrangements for sampling small quantities, e.g. flow-through microfluidic NMR chips · CPC title