Method for measuring blood coagulation

Patent metadata

Field	Value
Publication number	US-9733262-B2
Application number	US-201514658911-A
Country	US
Kind code	B2
Filing date	Mar 16, 2015
Priority date	May 20, 2009
Publication date	Aug 15, 2017
Grant date	Aug 15, 2017

Abstract

A method for measuring blood coagulation, including: preparing a reaction solution including a plasma specimen, a blood coagulation activator, and at least one of amino-guanidine and an acid addition salt thereof; and measuring blood coagulation time by detecting a turbidity change in the reaction solution.

First claim

The invention claimed is:

1. A method for measuring blood coagulation, comprising:
preparing a reaction solution comprising a plasma specimen, a blood coagulation activator, and at least one of amino-guanidine and an acid addition salt thereof; and
measuring blood coagulation time by detecting a turbidity change in the reaction solution.

2. The method according to claim 1 , wherein the turbidity change is detected by a method of detecting a scattered light amount.

3. The method according to claim 1 , wherein the amino-guanidine or an acid addition salt thereof is added to the reaction solution together with a specimen diluent for measuring blood coagulation.

4. The method according to claim 2 , wherein the amino-guanidine or an acid addition salt thereof is added to the reaction solution together with a specimen diluent for measuring blood coagulation.

5. The method according to claim 3 , wherein the specimen diluent is a specimen diluent for measuring fibrinogen.

6. The method according to claim 4 , wherein the specimen diluent is a specimen diluent for measuring fibrinogen.

7. The method according to claim 1 , wherein the blood coagulation activator is thrombin.

8. The method according to claim 5 , wherein the blood coagulation activator is thrombin.

9. The method according to claim 6 , wherein the blood coagulation activator is thrombin.

10. The method according to claim 5 , wherein the measuring of blood coagulation time is performed by a fibrinogen measurement method.

11. The method according to claim 6 , wherein the measuring of blood coagulation time is performed by a fibrinogen measurement method.

12. The method according to claim 7 , wherein the measuring of blood coagulation time is performed by a fibrinogen measurement method.

13. The method according to claim 9 , wherein the measuring of blood coagulation time is performed by a fibrinogen measurement method.

14. The method according to claim 9 , wherein the measuring of blood coagulation time is performed by a fibrinogen measurement method.

15. The method according to claim 1 , wherein the reaction solution comprises amino-guanidine.

16. The method according to claim 1 , wherein the reaction solution comprises an acid addition salt of amino-guanidine.

17. The method according to claim 1 , wherein the acid addition salt of amino-guanidine is at least one salt selected from the group consisting of a hydrochloride salt of amino-guanidine, a sulfate salt of amino-guanidine, a nitrate salt of amino-guanidine, a phosphate salt of amino-guanidine, and a sulfamate salt of amino-guanidine.

18. The method according to claim 1 , wherein the at least one of amino-guanidine and an acid addition salt thereof is amino-guanidine hydrochloride.

19. The method according to claim 1 , wherein the amino-guanidine has the formula

where R 1 is an amino group.

20. The method according to claim 17 , wherein the amino-guanidine has the formula

where R 1 is an amino group.

Assignees

• Sekisui Medical Co Ltd

Inventors

• Morikawa Chizuru
• Nakamura Remi
• Yamamoto Mitsuaki

Classifications

• C07C279/14

  being further substituted by carboxyl groups · CPC title

• A61P7/02

  Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors · CPC title

• C07C281/16

  Compounds containing any of the groups [IMAGE cpc-sch-C07C-0974.gif], e.g. aminoguanidine · CPC title

• G01N33/86 Primary

  involving blood coagulating time {or factors, or their receptors} · CPC title

• A61K31/155

  Amidines ([IMAGE cpc-sch-A61K-1029.gif]), e.g. guanidine (H2N—C(=NH)—NH2), isourea (N=C(OH)—NH2), isothiourea (—N=C(SH)—NH2) · CPC title