Biomarker for determining mitochondrial damage in friedreich's ataxia

Patent metadata

Field	Value
Publication number	US-9733258-B2
Application number	US-201314382367-A
Country	US
Kind code	B2
Filing date	Mar 1, 2013
Priority date	Mar 2, 2012
Publication date	Aug 15, 2017
Grant date	Aug 15, 2017

Abstract

Compositions and methods for screening for a disease or a disorder associated with a deficiency in frataxin in a subject using biomarkers for diseases or disorders associated with a deficiency in frataxin are disclosed. The compositions and methods include determining the acetylation status of mitochondrial proteins. Also disclosed are methods of detecting progression of a disease or a disorder associated with a deficiency in frataxin in a subject and methods of monitoring effectiveness of a therapy for diseases or disorders associated with a deficiency in frataxin.

First claim

What is claimed is:

1. A kit for measuring levels of mitochondrial protein acetylation comprising an anti-acetyl-lysine-specific antibody and an antibody that specifically binds to a mitochondrial protein wherein the mitochondrial protein is selected from the group consisting of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9 (NDUFA9), acetyl-CoA synthetase 2 (AceCS2), frataxin, complex II 30 kDa subunit, complex HI Rieske protein, NAD-dependent deacetylase sirtuin-3 (SIRT3), voltage-dependent anion-selective channel (VDAC), and combinations thereof.

2. The kit of claim 1 further comprising at least one of a reagent for processing a sample, a reagent for isolating mitochondria, and a reagent for measuring mitochondrial protein acetylation.

Assignees

• Univ Indiana Res & Tech Corp

Inventors

• Payne R Mark
• Wagner Gregory R
• Babbey Clifford M
• Pride P Melanie

Classifications

• G01N2440/10

  acylation, e.g. acetylation, formylation, lipoylation, myristoylation, palmitoylation · CPC title

• C12Q1/32

  involving dehydrogenase · CPC title

• G01N33/6893 Primary

  related to diseases not provided for elsewhere · CPC title

• G01N2500/02

  Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor) · CPC title

• G01N2800/52

  Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis · CPC title