Polypeptides having glucoamylase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9732332-B2
Application number	US-201013261653-A
Country	US
Kind code	B2
Filing date	Nov 30, 2010
Priority date	Nov 8, 2010
Publication date	Aug 15, 2017
Grant date	Aug 15, 2017

Abstract

The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

The invention claimed is:

1. A process of producing a fermentation product from starch-containing material comprising the steps of:
(1) liquefying starch-containing material;
(2) saccharifying the liquefied material; and
(3) fermenting with a fermenting organism;
wherein the fermentation product is ethanol, butanol or methanol, and step 2) is carried out using at least a polypeptide having glucoamylase activity selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2;
(b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the catalytic domain shown as amino acids 18 to 472 of SEQ ID NO: 2; and
(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

2. A process of producing a fermentation product from starch-containing material, wherein the fermentation product is ethanol, butanol or methanol, comprising the steps of:
(1) saccharifying starch-containing material at a temperature below the initial gelatinization temperature of said starch-containing material; and
(2) fermenting with a fermenting organism; wherein step (1) is carried out using at least a polypeptide having glucoamylase activity selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2;
(b) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the catalytic domain shown as amino acids 18 to 472 of SEQ ID NO: 2; and
(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1.

3. The process of claim 1 , wherein the polypeptide having glucoamylase activity has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

4. The process of claim 1 , wherein the polypeptide having glucoamylase activity has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2.

5. The process of claim 1 , wherein the polypeptide having glucoamylase activity has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2.

6. The process of claim 1 , wherein the polypeptide having glucoamylase activity has at least 97% sequence identity to the catalytic domain shown as amino acids 18 to 472 of SEQ ID NO: 2.

7. The process of claim 1 , wherein the polypeptide having glucoamylase activity has at least 98% sequence identity to the catalytic domain shown as amino acids 18 to 472 of SEQ ID NO: 2.

8. The process of claim 1 , wherein the polypeptide having glucoamylase activity has at least 99% sequence identity to the catalytic domain shown as amino acids 18 to 472 of SEQ ID NO: 2.

9. The process of claim 2 , wherein the polypeptide having glucoamylase activity has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2.

10. The process of claim 2 , wherein the polypeptide having glucoamylase activity has at least 98% sequence identity to the mature polypeptide of SEQ ID NO: 2.

11. The process of claim 2 , wherein the polypeptide having glucoamylase activity has at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 2.

12. The process of claim 2 , wherein the polypeptide having glucoamylase activity has at least 97% sequence identity to the catalytic domain shown as amino acids 18 to 472 of SEQ ID NO: 2.

13. The process of claim 2 , wherein the polypeptide having glucoamylase activity has at least 98% sequence identity to the catalytic domain shown as amino acids 18 to 472 of SEQ ID NO: 2.

14. The process of claim 2 , wherein the polypeptide having glucoamylase activity has at least 99% sequence identity to the catalytic domain shown as amino acids 18 to 472 of SEQ ID NO: 2.

15. The process of claim 1 , wherein the fermenting organism is a yeast.

16. The process of claim 15 , wherein the yeast is a Saccharomyces spp. strain.

17. The process of claim 16 , wherein the yeast is Saccharomyces cerevisiae.

18. The process of claim 2 , wherein the fermenting organism is a yeast.

19. The process of claim 18 , wherein the yeast is a Saccharomyces spp. strain.

20. The process of claim 19 , wherein the yeast is Saccharomyces cerevisiae.

Assignees

• Morant Marc D
• Sasa Mikako
• Ayabe Keiichi
• Coward-Kelly Guillermo
• Novozymes As
• Novozymes North America Inc

Inventors

• Morant Marc D
• Sasa Mikako
• Ayabe Keiichi
• Coward-Kelly Guillermo

Classifications

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12P19/14

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title

• C12P7/06

  Ethanol, i.e. non-beverage · CPC title

• Y02E50/17

  Cross-Sectional Technologies · mapped topic

• C12N9/2428 Primary

  Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase · CPC title