Polypeptide particle and method for producing same

Patent metadata

Field	Value
Publication number	US-9732125-B2
Application number	US-201414764472-A
Country	US
Kind code	B2
Filing date	Apr 18, 2014
Priority date	Apr 25, 2013
Publication date	Aug 15, 2017
Grant date	Aug 15, 2017

Abstract

Polypeptide particles of the present invention are particles of a polypeptide derived from spider silk proteins, and have an average particle size of 1000 nm or less. A method for producing polypeptide particles of the present invention includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from the group consisting of DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain an aqueous solution of the polypeptide; and a step in which the aqueous solution of the polypeptide is dried. Thereby, the present invention provides polypeptide particles suitable for application to a living body and capable of being applied to cosmetics, etc., while identifying the properties of the polypeptide particles, and a method for producing the same.

First claim

The invention claimed is:

1. Polypeptide particles that are particles of a polypeptide derived from spider silk proteins,
the polypeptide particles having an average particle size of 1000 nm or less,
wherein at least one selected from the group consisting of dimethyl sulfoxide and N, N-dimethylformamide is present inside the polypeptide particles.

2. The polypeptide particles according to claim 1 , wherein the polypeptide particles are spherical in shape.

3. A method for producing polypeptide particles, comprising:
a solution production step in which a polypeptide derived from spider silk proteins is dissolved in at least one dissolving solvent selected from the group consisting of: (A) dimethyl sulfoxide; (B) dimethyl sulfoxide with an inorganic salt; and (C) N, N-dimethylformamide with an inorganic salt, so as to obtain a solution of the polypeptide;
a step in which the solution of the polypeptide produced in the solution production step is substituted with a water-soluble solvent so as to obtain an aqueous solution of the polypeptide; and
a step in which the aqueous solution of the polypeptide is dried.

4. The method for producing polypeptide particles according to claim 3 , wherein the polypeptide particles are produced using an aqueous solution that is obtained by substituting the solution of the polypeptide with water.

5. The method for producing polypeptide particles according to claim 3 ,
wherein the substitution step using the water-soluble solvent is a step in which the solution of the polypeptide obtained by dissolving the polypeptide in the dissolving solvent is placed in a dialysis membrane,
the dialysis membrane is immersed in a water-soluble solvent, and
the water-soluble solvent is renewed at least one time.

6. The method for producing polypeptide particles according to claim 3 , wherein the drying is vacuum freeze-drying.

7. The polypeptide particles according to claim 1 , wherein the polypeptide particles can be dissolved in a water-soluble solvent or can be dispersed in a water-soluble solvent.

Assignees

• Spiber Inc

Inventors

• Osawa Toshiaki
• Morita Keisuke

Classifications

• C08J2389/00

  Characterised by the use of proteins; Derivatives thereof · CPC title

• A61K47/42

  Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein (oligopeptides having up to five amino acids {A61K47/183}; polyamino acids A61K47/34) · CPC title

• C07K14/43518 Primary

  from spiders · CPC title

• C08J3/07

  from polymer solutions · CPC title

• C08J3/12

  Powdering or granulating · CPC title