Methods for stepwise deposition of silk fibroin coatings

Patent metadata

Field	Value
Publication number	US-9731052-B2
Application number	US-201514705509-A
Country	US
Kind code	B2
Filing date	May 6, 2015
Priority date	Aug 2, 2005
Publication date	Aug 15, 2017
Grant date	Aug 15, 2017

Abstract

The invention provides a method for the controlled assembly of layered silk fibroin coatings using aqueous silk fibroin material. The methods described herein can be used to coat substrates of any material, shape, or size. Importantly, the described methods enable control of the biomaterial surface chemistry, thickness, morphology and structure using layered thin film coatings, or bulk coatings. Furthermore, the methods can be performed in all water and do not require intensive chemical processing enabling controlled entrapment of labile molecules such as, drugs, cytokines, and even cells or viruses to generate functional coatings that can be used in a variety of applications.

First claim

What is claimed is:

1. A composition comprising a substrate coated with at least one silk fibroin layer,
wherein the thickness of the at least one silk fibroin layer is from about 1 nm to about 12 nm,
wherein the at least one silk fibroin layer comprises a therapeutic agent, and
wherein the silk fibroin in the at least one silk fibroin layer comprises at least 40% beta sheet content.

2. The composition of claim 1 , wherein the composition is selected from the group consisting of vascular wound repair devices, hemostatic dressings, patches, glues, and sutures.

3. The composition of claim 1 , wherein the therapeutic agent comprises an agent for enhancing wound healing.

4. The composition of claim 1 , wherein the therapeutic agent is selected from the group consisting of antiproliferatives, antineoplastics, antiinflammatories, antiplatelets, anticoagulants, antifibrins, antithrombins, antimitotics, antibiotics, antioxidants, and combinations thereof.

5. The composition of claim 1 , wherein the composition is formulated for topical administration.

6. The composition of claim 1 , wherein the therapeutic agent is present as a liquid or a finely divided solid.

7. The composition of claim 1 , wherein the therapeutic agent is controlled released over a period of about 12 hours to about 90 days.

8. The composition of claim 1 , wherein the therapeutic agent is released in an amount of about 1 ng/day to 1 mg/day.

9. The composition of claim 1 , wherein composition further comprises an excipient.

10. The composition of claim 1 , wherein the substrate is coated with at least two silk fibroin layers.

11. The composition of claim 10 , wherein one or more of the at least two silk fibroin layers comprise no added therapeutic agent.

12. The composition of claim 11 , wherein an outermost silk fibroin layer comprises no added therapeutic agent.

13. The composition of claim 10 , wherein one or more of the at least two silk fibroin layers comprise different therapeutic agents than one or more other silk fibroin layers.

14. The composition of claim 10 , wherein one or more of the at least two silk fibroin layers comprises different concentrations of therapeutic agent than one or more other silk fibroin layers.

15. The composition of claim 1 , wherein the substrate is selected from the group consisting of plastic, wood, glass, leather, cloth, synthetic fibers, ceramic, and metal or alloy.

16. The composition of claim 1 , wherein the substrate is selected from the group consisting of sutures, meshes, plates, screws, cements, biomedical devices, biomaterials, biosensors, tissue engineering scaffolds, stents, tubing, gels, and 3D porous scaffolds.

17. The composition of claim 1 , wherein said at least one silk fibroin layer further comprises a biocompatible polymer.

Assignees

• Tufts College

Inventors

• Kaplan David L
• Wang Xianyan

Classifications

• C09D189/00

  Coating compositions based on proteins; Coating compositions based on derivatives thereof (foodstuff preparations A23J3/00) · CPC title

• A61P43/00

  Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title

• A61L27/50

  Materials characterised by their function or physical properties {, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials} · CPC title

• A61L15/44

  Medicaments · CPC title

• A61L15/32

  Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin {(A61L15/225 takes precedence)} · CPC title