Polynucleotide encoding NF-YB derived from jatropha and use thereof

Patent metadata

Field	Value
Publication number	US-9725733-B2
Application number	US-201615235289-A
Country	US
Kind code	B2
Filing date	Aug 12, 2016
Priority date	Apr 9, 2010
Publication date	Aug 8, 2017
Grant date	Aug 8, 2017

Abstract

By analyzing a Jatropha genome, NF-YB-encoding genes of SEQ ID NOs: 1 to 11, fragments of NF-YB-encoding genes of SEQ ID NOs: 12 and 13, and genes relating thereto were found. By transforming Jatropha with these NF-YB-encoding genes and the like, it is possible to overexpress a NF-YB polypeptide and so on, and to significantly improve the productivity of protein synthesis involved by the NF-YB polypeptide, and to significantly improve the dry stress resistance, for example. As a result, it is possible to create dry stress resistant Jatropha capable of ensuring high growth even under water deficient conditions.

First claim

The invention claimed is:

1. A vector comprising a heterologous nucleotide sequence and a polynucleotide with at least 99% identity to SEQ ID NO:6, wherein expression of the polynucleotide enhances stress resistance in Jatropha compared to wild-type Jatropha.

2. The vector according to claim 1 , comprising SEQ ID NO:6.

3. The vector according to claim 1 , which is a Jatropha plant transformation vector.

4. A transgenic Jatropha plant containing the vector according to claim 3 .

5. A transgenic Jatropha plant transformed with the vector according to claim 3 , wherein the transgenic Jatropha plant has increased stress resistance compared to a corresponding control Jatropha plant.

6. A method for preparing a stress resistant Jatropha plant, said method comprising: preparing an isolated polynucleotide with at least 99% identity to SEQ ID NO:6,
wherein expression of the polynucleotide enhances stress resistance in Jatropha compared to wild-type Jatropha ;
preparing a Jatropha plant transformation vector comprising said isolated polynucleotide;
and transforming a Jatropha plant with said vector.

Assignees

• Univ Osaka

Inventors

• Matsunaga Sachihiro
• Kohinata Tsutomu
• Fukui Kiichi
• Tabata Satoshi

Classifications

• C12N15/8273 Primary

  for drought, cold, salt resistance · CPC title

• C07K14/415

  from plants · CPC title