Enzymes for starch processing

Patent metadata

Field	Value
Publication number	US-9719120-B2
Application number	US-201414460938-A
Country	US
Kind code	B2
Filing date	Aug 15, 2014
Priority date	Dec 22, 2004
Publication date	Aug 1, 2017
Grant date	Aug 1, 2017

Abstract

The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.

First claim

The invention claimed is:

1. A process comprising:
(a) contacting a starch substrate with a yeast cell transformed to express a polypeptide comprising a first amino acid sequence comprising a catalytic module having alpha-amylase activity and a second amino acid sequence comprising a carbohydrate-binding module, wherein the first amino acid sequence has at least 90% sequence identity to SEQ ID NO: 24, and wherein the second amino acid sequence has at least 90% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 92, SEQ ID NO: 94 and SEQ ID NO: 96;
(b) holding said starch substrate with said yeast; and
(c) fermenting to produce ethanol,
wherein steps a, b, and c are performed separately or simultaneously.

2. The process of claim 1 , wherein the process further comprises the step of recovering ethanol.

3. The process of claim 1 , wherein the first amino acid sequence has at least 95% sequence identity to SEQ ID NO: 24.

4. The process of claim 1 , wherein the second amino acid sequence has at least 95% sequence identity to SEQ ID NO: 92.

5. The process of claim 1 , wherein the second amino acid sequence has at least 95% sequence identity to SEQ ID NO: 94.

6. The process of claim 1 , wherein the second amino acid sequence has at least 95% sequence identity to SEQ ID NO: 96.

7. A process comprising:
(a) contacting a starch substrate with a yeast cell transformed to express a polypeptide comprising a first amino acid sequence comprising a catalytic module having alpha-amylase activity and a second amino acid sequence comprising a carbohydrate-binding module, wherein the first amino acid sequence has at least 95% sequence identity to SEQ ID NO: 24, and wherein the second amino acid sequence has at least 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 92, SEQ ID NO: 94 and SEQ ID NO: 96;
(b) holding said starch substrate with said yeast; and
(c) fermenting to produce alcohol,
wherein steps a, b, and c are performed separately or simultaneously.

Assignees

• Novozymes As
• Novozymes North America Inc

Inventors

• Fukuyama Shiro
• Matsui Tomoko
• Soong Chee Leong
• Allain Eric
• Nielsen Anders Vikso
• Udagawa Hiroaki
• Liu Ye
• Duan Junxin
• Wu Wenping
• Andersen Lene Nonboe

Classifications

• Y02E50/16

  Cross-Sectional Technologies · mapped topic

• C07K2319/00

  Fusion polypeptide · CPC title

• C12N9/2417

  from microbiological source · CPC title

• C12N9/96

  Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title