Method for monitoring level of paraben in cosmetics

Patent metadata

Field	Value
Publication number	US-9715998-B2
Application number	US-201414646983-A
Country	US
Kind code	B2
Filing date	Jan 9, 2014
Priority date	Jan 9, 2014
Publication date	Jul 25, 2017
Grant date	Jul 25, 2017

Abstract

The invention discloses a method for monitoring level of paraben comprising: dissolving a sample in a solvent and obtaining a supernatant containing paraben by ultrasonic vibration and high speed centrifugation; performing a derivatization reaction between a derivatization reagent and paraben by adding the derivatization reagent into the supernatant to obtain a derivatization solution containing a tagged paraben; extracting the derivatization solution by an extractant to obtain an extract containing the tagged paraben; and ionizating the tagged paraben by a laser beam and analyzing mass-to-charge ratio of the tagged paraben by an analyzer to determine molecular weight thereof.

First claim

What is claimed is:

1. A method for monitoring level of paraben in cosmetics comprising:
dissolving a sample in a solvent and obtaining a supernatant containing paraben by ultrasonic vibration and high speed centrifugation;
performing a derivatization reaction between a derivatization reagent and paraben by adding the derivatization reagent into the supernatant containing paraben to obtain a derivatization solution containing a tagged paraben;
extracting the derivatization solution containing the tagged paraben with an extractant to obtain an extract containing the tagged paraben;
co-crystallizing the extract containing the tagged paraben and α-cyanol-4-hydroxycinnamic acid (CHCA) as a matrix to form a co-crystallized extract-matrix mixture;
ionizing the co-crystallized extract-matrix mixture by a laser beam having a wavelength of 330 to 360 nm to form a vaporized ion; and
analyzing mass-to-charge ratio of the vaporized ion with an analyzer to determine molecular weight thereof.

2. The method for monitoring level of paraben in cosmetics as claimed in claim 1 , wherein the method further comprises adjusting temperature of the supernatant containing paraben to 30-80° C. prior to adding the derivatization reagent into the supernatant containing paraben.

3. The method for monitoring level of paraben in cosmetics as claimed in claim 1 , wherein the derivatization reagent has a sulfonyl chloride group, wherein the derivatization reaction is performed with a hydroxyl group of paraben.

4. The method for monitoring level of paraben in cosmetics as claimed in claim 3 , wherein the derivatization reagent is selected from 1,3-benzothiazole-6-sulfonyl chloride, 4-dimethylaminoazobenzene-4′-sulfonyl chloride or dansyl chloride.

5. The method for monitoring level of paraben in cosmetics as claimed in claim 1 , wherein the sample is in a form of solution, lotion, emulsion, cream or gel.

6. The method for monitoring level of paraben in cosmetics as claimed in claim 1 , wherein the method further comprises adding an internal standard into the supernatant containing paraben, and performing the derivatization reaction between the derivatization reagent and the internal standard.

7. The method for monitoring level of paraben in cosmetics as claimed in claim 6 , wherein the internal standard is an isotope of paraben.

Assignees

• Univ Kaohsiung Medical
• Kaohsuing Medical Univ

Inventors

• Lu Chi-Yu
• Lee Yi-Hsuan

Classifications

• H01J49/164 Primary

  Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI] (sample holders H01J49/0418) · CPC title

• H01J49/0036 Primary

  Step by step routines describing the handling of the data generated during a measurement · CPC title