Bio-sensing nanodevice

Patent metadata

Field	Value
Publication number	US-9714941-B2
Application number	US-18391608-A
Country	US
Kind code	B2
Filing date	Jul 31, 2008
Priority date	Jul 31, 2007
Publication date	Jul 25, 2017
Grant date	Jul 25, 2017

Abstract

The invention provides a bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—on a support, a real time receptor-ligand binding detection method, an odorant delivery system and an odorant recognition program. The biologically-derived G-protein coupled receptor can be stabilized on nanotechnology using surfactant peptide. The said nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor.

First claim

What is claimed is:

1. A bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled olfactory receptor-immobilized on a support, a real time receptor-ligand binding detection system method, a test composition delivery system and a test composition recognition program, wherein the stabilized receptor is stabilized with surfactant peptides selected from the group consisting of:

Sequence (N → C)
Formula

(φ) m (+) n
1

(+) n (φ) m
2

(φ) m (−) n
3

(−) n (φ) m
4

(−) n (φ) m (−) n
5

(+) n (φ) m (+) n
6

(φ) m (−)n(φ) m
7

(φ) m (+)n(φ) m
8

(+) n (φ) m (−) n
9

(−) n (φ) m (+) n
10

wherein:
(Φ) represents independently for each occurrence a natural or non-natural amino acid comprising a hydrophobic sidechain;
(+) represents independently for each occurrence a natural or non-natural amino acid comprising a sidechain that is cationic at physiological pH;
(−) represents independently for each occurrence a natural or non-natural amino acid comprising a sidechain that is anionic at physiological pH;
wherein the terminal amino acids are optionally substituted;
m for each occurrence represents an integer greater than or equal to 5; and
n for each occurrence represents an integer greater than or equal to 1;

wherein the test composition delivery system is a microfluidic bubble logic operation.

2. The bio-sensing nanodevice of claim 1 , wherein the microfluidic bubble logic operation comprises microfluidic channels adapted for receiving micron-sized droplets and bubbles comprising a test composition.

Assignees

• Zhang Shuguang
• Mershin Andreas
• Kaiser Liselotte
• Cook Brian
• Graveland-Bikker Johanna F
• Prakash Manu
• Kong David
• Maguire Yael
• Massachusetts Inst Technology

Inventors

• Zhang Shuguang
• Mershin Andreas
• Kaiser Liselotte
• Cook Brian
• Graveland-Bikker Johanna F
• Prakash Manu
• Kong David
• Maguire Yael

Classifications

• G01N2333/726

  G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH · CPC title

• G01N33/54366 Primary

  Apparatus specially adapted for solid-phase testing · CPC title