System and method for cell levitation and monitoring
US-2024361343-A1 · Oct 31, 2024 · US
US9714934B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9714934-B2 |
| Application number | US-201113817976-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 22, 2011 |
| Priority date | Aug 20, 2010 |
| Publication date | Jul 25, 2017 |
| Grant date | Jul 25, 2017 |
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A multi-phase system includes a phase-separated solution comprising at least two phases, each phase having a phase component selected from the group consisting of a polymer, a surfactant and combinations thereof, wherein at least one phase comprises a polymer, wherein the phases, taken together, represent a density gradient. Novel two-phase, three-phase, four-phase, five-phase, or six-phase systems are disclosed. Using the disclosed multi-phase polymer systems, particles, or other analyte of interest can be separated based on their different densities or affinities.
Opening claim text (preview).
What is claimed is: 1. An aqueous two-phase system, comprising: a phase-separated solution comprising two aqueous phases, wherein each of the phases comprises a phase component and has a different density and the phases, taken together, represent a density gradient, and wherein the phase component combination of the two phases is selected from the group consisting of: Number Phase component combinations 1 poly(2-ethyl-2-oxazoline) poly(methacrylic acid) 2 poly(2-ethyl-2-oxazoline) poly(vinyl alcohol) 3 poly(ethylene glycol) poly(methacrylic acid) 6 poly(ethylene glycol) poly(2-ethyl-2-oxazoline) 8 dextran poly(2-ethyl-2-oxazoline) 10 Ficoll poly(methacrylic acid) 11 Ficoll poly(vinyl alcohol) 12 Ficoll poly(2-ethyl-2-oxazoline) 15 polyacrylamide poly(methacrylic acid) 18 polyacrylamide poly(2-ethyl-2-oxazoline) 20 poly(diallyldimethyl ammonium chloride poly(methacrylic acid) 21 poly(diallyldimethyl ammonium chloride poly(acrylic acid) 22 poly(diallyldimethyl ammonium chloride poly(vinyl alcohol) 23 poly(diallyldimethyl ammonium chloride poly(2-ethyl-2-oxazoline) 24 poly(diallyldimethyl ammonium chloride poly(ethylene glycol) 25 dextran sulfate poly(vinyl alcohol) 26 dextran sulfate poly(2-ethyl-2-oxazoline) 28 chondroitin sulfate A poly(methacrylic acid) 29 chondroitin sulfate A poly(vinyl alcohol) 30 chondroitin sulfate A poly(2-ethyl-2-oxazoline) 31 polyethyleneimine poly(methacrylic acid) 32 polyethyleneimine poly(2-ethyl-2-oxazoline) 34 polyethyleneimine Ficoll 35 polyethyleneimine polyacrylamide 36 polyvinylpyrrolidone poly(methacrylic acid) 39 poly(propylene glycol) poly(methacrylic acid) 41 poly(propylene glycol) polyacrylamide 42 poly(2-acrylamido-2-methyl-1-propanesulfonic acid) dextran 43 poly(2-acrylamido-2-methyl-1-propanesulfonic acid) polyvinylpyrrolidone 44 poly(styrene sulfonic acid) poly(2-ethyl-2-oxazoline) 45 poly(styrene sulfonic acid) dextran sulfate 46 diethylaminoethyl-dextran poly(acrylic acid) 47 polyallylamine dextran sulfate 48 alginic acid poly(acrylic acid) 49 alginic acid poly(propylene glycol) 50 (hydroxypropyl)methyl cellulose poly(diallyldimethyl ammonium chloride 51 (hydroxypropyl)methyl cellulose poly(propylene glycol) 52 carboxy-polyacrylamide poly(methacrylic acid) 53 carboxy-polyacrylamide poly(vinyl alcohol) 54 carboxy-polyacrylamide polyethyleneimine 55 hydroxyethyl cellulose dextran 56 hydroxyethyl cellulose Ficoll 57 methyl cellulose Ficoll 58 Zonyl poly(methacrylic acid) 59 Zonyl dextran 60 Zonyl polyacrylamide 61 Brij poly(2-ethyl-2-oxazoline) 62 Brij Ficoll 63 Brij polyallylamine 64 Tween poly(methacrylic acid) 65 Tween poly(vinyl alcohol) 66 Tween poly(2-ethyl-2-oxazoline) 69 Tween Ficoll 71 Tween polyallylamine 72 Tween hydroxyethyl cellulose 73 Triton poly(methacrylic acid) 74 Triton poly(acrylic acid) 75 Triton poly(2-ethyl-2-oxazoline) 77 Triton Ficoll 78 Triton polyacrylamide 79 Triton polyallylamine
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