Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system

Patent metadata

Field	Value
Publication number	US-9714439-B2
Application number	US-201615184242-A
Country	US
Kind code	B2
Filing date	Jun 16, 2016
Priority date	Feb 10, 2010
Publication date	Jul 25, 2017
Grant date	Jul 25, 2017

Abstract

The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.

First claim

What is claimed is:

1. A process for converting a compound mixture of formula IId which comprises a substrate for an amino acid dehydrogenase to a composition of formula I and a chiral amino acid of formula IIa:

comprising contacting the compound mixture of formula IId with an enantioselective amino acid L-leucine dehydrogenase in a reaction medium comprising NAD + /NADH or NADP + /NADPH and a cofactor recycling system comprising a ketoreductase comprising the amino acid sequence of SEQ ID NO: 18, and a lower alkyl ketone, under conditions where the compound mixture of formula IId is converted to the composition of formula I and a chiral amino acid of formula IIa, and the lower alkyl ketone is converted to a lower secondary alcohol; wherein R is a substituted or unsubstitued —(C 1 -C 10 )alkyl, —(C 2 -C 6 )alkenyl, —(C 2 -C 6 )alkynl, —(C 3 -C 8 )cycloalkyl, heterocycloalky, aryl, or heteroaryl.

2. The process of claim 1 , wherein the compound mixture of IId is a racemic mixture of formula IIe:

3. The process of claim 1 , wherein the amino acid dehydrogenase comprises an L-amino acid dehydrogenase and the chiral amino acid of formula IIa is IIc

wherein the chiral amino acid of formula IIc is present in enantiomeric excess.

4. The process of claim 1 , wherein the L-leucine dehydrogenase is from Bacillus, Clostridium, Corynebacterium, Geobacillus, Natronobacterium, Synechocystis, Thermoactinomyces, Thermos, Thermomicrobium , or Carderia.

5. The process of claim 1 which is carried out in a cell free system.

6. The process of claim 1 , wherein the amino acid dehydrogenase is present as a crude extract.

7. The process of claim 1 , wherein the amino acid dehydrogenase is substantially purified.

8. The process of claim 1 , wherein the ketoreductase is a wild type ketoreductase or an engineered ketoreductase.

9. The process of claim 8 , wherein the ketoreductase is from Lactobacillus, Candida, Novosphingobium , or Saccharomyces.

10. The process of claim 9 , wherein the ketoreductase of Lactobacillus is from Lactobacillus kefir, Lactobacillus brevis , or Lactobacillus minor.

11. The process of claim 9 , wherein the ketoreductase of Candida is from Candida magnoliae.

12. The process of claim 9 , wherein the ketoreductase of Saccharomyces is from Saccharomyces cerevisiae.

13. The process of claim 9 , wherein the ketoreductase of Novosphingobium is from Novosphingobium aromaticivorans.

14. The process of claim 1 , wherein the ketoreductase is an engineered ketoreductase characterized by increased thermostability, increased solvent stability, and/or increased enzymatic activity relative to the wild type ketoreductase.

15. The process of claim 1 , wherein the ketoreductase is present as a crude extract.

16. The process of claim 1 , wherein the ketoreductase is substantially purified.

17. The process of claim 1 , further comprising the step of removing the lower secondary alcohol formed from the lower alkyl ketone from the reaction medium.

18. The process of claim 1 , wherein the lower alkyl ketone is acetone and the lower secondary alcohol is isopropanol.

19. The process of claim 18 , wherein the isopropanol is removed from the reaction medium.

20. The process of claim 1 , wherein the reaction medium is at a pH of about 8.5 to about 10.

21. The process of claim 20 , wherein the reaction medium is at a pH of about 8.5 to about 9.0.

22. The process of claim 1 , wherein the reaction medium is at a temperature of about 25° C. to about 45° C.

23. The process of claim 22 , wherein the reaction medium is at a temperature of about 35° C. to about 40° C.

24. The process of claim 1 , wherein the lower alkyl ketone is present in at least 1.5 fold stoichiometric excess of the substrate of formula IIc.

Assignees

• Codexis Inc

Inventors

• Cabirol Fabien Louis
• Collier Steven J
• Daussmann Thomas
• Modukuru Naga K

Classifications

• C12N9/0004

  Oxidoreductases (1.) · CPC title

• C12P7/40

  containing a carboxyl group {including Peroxycarboxylic acids} · CPC title

• C12N9/0016

  with NAD or NADP as acceptor (1.4.1) · CPC title

• C12Y104/99001

  D-Amino-acid dehydrogenase (1.4.99.1) · CPC title

• C12Y101/00

  Oxidoreductases acting on the CH-OH group of donors (1.1) · CPC title