Cellulolytic enzyme compositions and uses thereof

Patent metadata

Field	Value
Publication number	US-9714416-B2
Application number	US-201214343002-A
Country	US
Kind code	B2
Filing date	Sep 21, 2012
Priority date	Sep 23, 2011
Publication date	Jul 25, 2017
Grant date	Jul 25, 2017

Abstract

The present invention relates enzyme composition comprising a cellulolytic preparation and an acetylxylan esterase (AXE); and the used of cellulolytic enzyme compositions for hydrolyzing acetylated cellulosic material. Finally the invention also relates to processes of producing fermentation products from acetylated cellulosic materials using a cellulolytic enzyme composition of the invention.

First claim

The invention claimed is:

1. A method of hydrolyzing acetylated acid-pretreated cellulosic material, comprising subjecting the acetylated cellulosic material to a cellulolytic preparation and an acetylxylan esterase (AXE),
wherein the acetylxylan esterase (AXE) is obtained from a strain of Aspergillus aculaetus and has at least 80% sequence identity to SEQ ID NO: 2.

2. The method of claim 1 , wherein the acetylated acid-pretreated cellulosic material is thermomechanically pulped plant material.

3. The method of claim 1 , wherein the cellulolytic preparation is a Trichoderma reesei cellulolytic preparation, further comprising Penicillium emersonii GH61A polypeptide having cellulolytic enhancing activity, Aspergillus fumigatus beta-glucosidase variant and Aspergillus fumigatus beta-xylosidase.

4. The method of claim 1 , wherein the ratio between cellulolytic preparation and acetylxylan esterase (AXE) is in the range in a ratio of between 500:1 and 1:1, between 50:1 and 2:1, or about 4:1.

5. A process of producing a fermentation product from acetylated acid-pretreated cellulosic material, comprising:
(a) hydrolyzing said acetylated acid-pretreated cellulosic material by subjecting the material to an enzyme composition comprising a cellulolytic preparation and an acetylxylan esterase (AXE),
wherein the acetylxylan esterase (AXE) is obtained from a strain of Aspergillus aculaetus and has at least 80% sequence identity to SEQ ID NO: 2;
(b) fermenting using a fermenting organism; and
(c) optionally recovering the fermentation product.

6. The process of claim 5 , wherein the fermentation product is ethanol.

7. The process of claim 5 , wherein the acetylated acid-pretreated cellulosic material is corn stover pulp.

8. The method of claim 2 , wherein the thermomechanically pulped plant material is acetylated corn stover pulp.

9. The method of claim 1 , wherein the acetylated acid-pretreated cellulosic material has a degree of acetylation of the cellulosic material of about 5-10%.

10. The method of claim 5 , wherein the acetylated acid-pretreated cellulosic material has a degree of acetylation of the cellulosic material of about 5-10%.

11. The method of claim 1 , wherein the acetylxylan esterase has at least 85% sequence identity to SEQ ID NO: 2.

12. The method of claim 1 , wherein the acetylxylan esterase has at least 90% sequence identity to SEQ ID NO: 2.

13. The method of claim 1 , wherein the acetylxylan esterase has at least 95% sequence identity to SEQ ID NO: 2.

14. The method of claim 1 , wherein the acetylxylan esterase has at least 96% sequence identity to SEQ ID NO: 2.

15. The method of claim 1 , wherein the acetylxylan esterase has at least 97% sequence identity to SEQ ID NO: 2.

16. The method of claim 1 , wherein the acetylxylan esterase has at least 98% sequence identity to SEQ ID NO: 2.

17. The method of claim 1 , wherein the acetylxylan esterase has at least 99% sequence identity to SEQ ID NO: 2.

18. The method of claim 5 , wherein the acetylxylan esterase has at least 85% sequence identity to SEQ ID NO: 2.

19. The method of claim 5 , wherein the acetylxylan esterase has at least 90% sequence identity to SEQ ID NO: 2.

20. The method of claim 5 , wherein the acetylxylan esterase has at least 95% sequence identity to SEQ ID NO: 2.

21. The method of claim 5 , wherein the acetylxylan esterase has at least 96% sequence identity to SEQ ID NO: 2.

22. The method of claim 5 , wherein the acetylxylan esterase has at least 97% sequence identity to SEQ ID NO: 2.

23. The method of claim 5 , wherein the acetylxylan esterase has at least 98% sequence identity to SEQ ID NO: 2.

24. The method of claim 5 , wherein the acetylxylan esterase has at least 99% sequence identity to SEQ ID NO: 2.

Assignees

• Novozymes As

Inventors

• Iyer Prashant
• Gaspar Armindo Ribiero
• Croonenberghs James
• Binder Thomas P

Classifications

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C07K14/37 Primary

  from fungi · CPC title

• C12N9/18 Primary

  Carboxylic ester hydrolases {(3.1.1)} · CPC title

• Y02E50/16

  Cross-Sectional Technologies · mapped topic

• C12N9/2445

  Beta-glucosidase (3.2.1.21) · CPC title