MALDI/SELDI analysis of complex mixtures comprising hydrophilic and hydrophobic analytes

Patent metadata

Field	Value
Publication number	US-9709583-B2
Application number	US-200913060249-A
Country	US
Kind code	B2
Filing date	Aug 20, 2009
Priority date	Aug 20, 2008
Publication date	Jul 18, 2017
Grant date	Jul 18, 2017

Abstract

The present disclosure relates to the identification of components in a sample based on the chemical nature of the component. In particular, although not exclusively, the present disclosure relates to the identification of hydrophobic and hydrophilic components using a mass spectrometric technique. The present invention also relates to kits and products used in the identification of the components, as well as other subject matter.

First claim

The invention claimed is:

1. A method of preparing and analyzing a complex liquid sample comprising a hydrophilic target constituent and a hydrophobic target constituent, comprising:
mixing the sample with hydrophilic and hydrophobic silica particles on a plate suitable for MALDI/SALDI analysis to form a first complex between the hydrophilic target constituent and the hydrophilic silica particles and a second complex between the hydrophobic target constituent and the hydrophobic silica particles, wherein either the hydrophilic or the hydrophobic silica particles are functionalized to include a magnetic component;
separating the first complex from the second complex by selectively positioning at least one magnet with respect to at least one of the first and the second complex and removing one complex from another directly on the plate using the at least one magnet; and
carrying out a MALDI/SALDI analysis on the separated first complexes, without further addition of a citrate or a MALDI/SALDI matrix,
wherein the sample is not washed or rinsed after the separation and before carrying out the MALDI/SALDI analysis.

2. The method of claim 1 , wherein the hydrophobic silica particles are formed from silane ether monomers and organically modified silane ether monomers in the presence of a hydrolysing agent.

3. The method of claim 1 , wherein the hydrophilic silica particles are formed from 3-aminopropyltriethoxysilane (APTES) monomers.

4. The method of claim 1 , wherein the hydrophilic silica particles and hydrophobic silica particles are mixed together in a suspension and applied to the sample, wherein the suspension is an ethanolic suspension.

5. The method of claim 1 , wherein the hydrophobic silica particles are embedded with carbon black.

6. The method of claim 1 , wherein the hydrophilic silica particles are embedded with carbon black.

7. The method of claim 1 , wherein the MALDI/SALDI analysis is selected from MALDI-TOF-MS and SALDI-TOF-MS.

8. The method of claim 1 , wherein the hydrophilic or the hydrophobic target constituent is selected from the group consisting of a protein, DNA, RNA, a peptide, an amino acid, and an organic compound.

9. The method of claim 1 , wherein the sample comprises a fingerprint, a footprint, an earprint, or any residue left on a surface following contact between the surface and a part of a person's skin.

Assignees

• Rowell Frederick
• Ma Jan
• Lim Angelina Yi Mei
• Univ Nanyang Tech

Inventors

• Rowell Frederick
• Ma Jan
• Lim Angelina Yi Mei

Classifications

• G01N1/10

  in the liquid or fluent state {(burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00)} · CPC title

• G01N1/04

  in the solid state, e.g. by cutting · CPC title

• Y10T436/24

  Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry · CPC title

• G01N1/18

  with provision for splitting samples into portions (G01N1/12, G01N1/14 take precedence; fraction-collection apparatus for chromatography B01D15/08) · CPC title

• G01N33/6848

  Methods of protein analysis involving mass spectrometry · CPC title