Anthraquinone based near IR emitting compounds and uses thereof

Patent metadata

Field	Value
Publication number	US-9709557-B2
Application number	US-201514632168-A
Country	US
Kind code	B2
Filing date	Feb 26, 2015
Priority date	Oct 27, 2008
Publication date	Jul 18, 2017
Grant date	Jul 18, 2017

Abstract

Disclosed are near IR emitting fluorescent compounds; methods of making and kits containing the described compounds; and their use in fluorescence-based detection of biological materials.

First claim

What is claimed is:

1. A method of staining a biological sample containing nucleic acid, comprising:
a) contacting the biological sample with a compound having the structure:

wherein R 2 , R 3 , and R 4 are independently OH or NHR 9 ,
wherein R 9 is H or (CH 2 ) n COR 5 and
R 1 is —(CH 2 ) n COR 5 ,
wherein R 5 is NH(CH 2 ) m NR 7 R 8 or NH(CH 2 ) m N + R 7 R 8 R 10 ,
wherein R 7 , R 8 and R 10 are independently H or C 1-10 alkyl, m is 2 to 10, and n is 1 to 5 to form a mixture;
b) incubating the mixture for a sufficient amount of time for the compound to associate with nucleic acid in the sample to form an incubated sample;
c) exciting the incubated sample by illumination with light of an appropriate wavelength to generate a detectable optical response; and
d) detecting the optical response of the compound.

2. The method of claim 1 wherein the biological sample comprises cells.

3. The method of claim 2 wherein the cells are treated with a cell fixative reagent, or a cell fixative reagent combined with a cell permeabilizing reagent, or a cell permeabilizing reagent.

4. The method of claim 1 wherein the biological sample comprises fixed cells, or fixed and permeabilized cells, fixed and permeabilized cells, or free cell nuclei.

5. The method of claim 1 wherein the biological sample comprises live cells.

6. The method of claim 1 , wherein said compound is in solution.

Assignees

• Life Technologies Corp

Inventors

• Yue Stephen
• Huang Shih-Jung
• Bradford Jolene

Classifications

• G01N21/6428

  Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53) · CPC title

• G01N33/52 Primary

  Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper {and including single- and multilayer analytical elements (immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72)} · CPC title

• G01N2021/6441

  with two or more labels · CPC title

• C12Q1/6816 Primary

  characterised by the detection means (C12Q1/6804 takes precedence) · CPC title

• C09B1/5165

  only amino and hydroxy groups · CPC title