Nucleic acid preparation method

Patent metadata

Field	Value
Publication number	US-9708645-B2
Application number	US-201113989782-A
Country	US
Kind code	B2
Filing date	Nov 28, 2011
Priority date	Nov 26, 2010
Publication date	Jul 18, 2017
Grant date	Jul 18, 2017

Abstract

A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support while maintaining it on the support. The polyol may for example be a C 2 -C 10 alkanediol.

First claim

The invention claimed is:

1. A method for processing a nucleic acid comprising:
exposing the nucleic acid, wherein the nucleic acid is dissolved in a solution, to a polyol or a liquid comprising the polyol without the use of a monohydric alcohol to induce or facilitate at least a portion of the dissolved nucleic acid to enter an extra-solution phase wherein the polyol is selected from 2-methyl-1,3-propanediol, 2,3-butanediol, and tripropyleneglycol.

2. The method of claim 1 , wherein the extra-solution phase is a solid support-bound phase or the extra-solution phase comprises beads capable of binding nucleic acid.

3. The method of claim 2 , further comprising a method for washing solid support-bound nucleic acid or bead-bound nucleic acid, wherein the method comprises:
contacting the solid support or bead and its bound nucleic acid with the polyol or the liquid comprising the polyol; and
optionally recovering the solid support or bead and its bound nucleic acid from the liquid.

4. The method of claim 1 , wherein the liquid is an aqueous medium comprising at least about 10% v/v of the polyol.

5. The method of claim 1 , wherein the polyol has a flashpoint of at least 50° C. or at least 70° C.

6. The method of claim 1 , wherein the liquid is an aqueous medium comprising at least about 25% v/v of the polyol.

7. The method of claim 1 , wherein the liquid is an aqueous medium comprising at least about 40% v/v of the polyol.

8. The method of claim 1 , wherein the liquid is an aqueous medium comprising at least about 50% v/v of the polyol.

9. The method of claim 1 , wherein the liquid is an aqueous medium comprising at least about 60% v/v of the polyol.

10. The method of claim 1 , wherein the polyol is 2-methyl-1,3-propanediol.

Assignees

• Ellis Darren
• Fonnum Geir
• Kjus Nini Hofslokken
• Life Technologies Corp

Inventors

• Ellis Darren
• Fonnum Geir
• Kjus Nini Hofslokken

Classifications

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C12N15/1013

  by using magnetic beads · CPC title

• C12N15/1006

  by means of a solid support carrier, e.g. particles, polymers · CPC title