Simultaneous acquisition of biometric data and nucleic acid

Patent metadata

Field	Value
Publication number	US-9708644-B2
Application number	US-201313774903-A
Country	US
Kind code	B2
Filing date	Feb 22, 2013
Priority date	Feb 22, 2012
Publication date	Jul 18, 2017
Grant date	Jul 18, 2017

Abstract

Systems, methods, and kits are disclosed for collection, labeling and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of an individual. Such devices and methods are used in forensic, human identification, access control and screening technologies to rapidly process an individual's identity or determine the identity of an individual.

First claim

What is claimed:

1. A device comprising:
a collection component; and
a handling component;
wherein the collection component is detachably connected to the handling component; and
wherein the collection component comprises an active collection surface area encompassing an exterior edge of an angled fold of the collection component, the angled fold formed over a first portion of the handling component
and wherein the active collection surface area:
a) can collect a biological sample from an appendage placed on at least a first surface area of a substrate made of a polymeric film or a glass, wherein the active collection surface area is at least 50% smaller than the first surface area of the substrate; and
b) allows direct nucleic acid amplification reaction of the biological sample collected in the active collection surface area or a portion thereof.

2. The device of claim 1 , wherein a portion of the active collection surface area can be punched out and used for archiving.

3. The device of claim 1 , wherein the collection component comprises fibrous material and wherein the fibrous material comprises natural fibers, synthetic polymeric fibers or a combination thereof.

4. The device of claim 3 , wherein the fibrous material is cotton, paper, nitrocellulose paper, nylon paper, cellulose paper, cotton paper, polyester papers, and combinations thereof and optionally, wherein the fibrous material is chemically treated.

5. The device of claim 1 , wherein the first portion of the handling component further comprises a layer of absorbent material underlying the collection component.

6. The device of claim 1 , wherein the first portion of the handling component comprises a stiffening support for the collection component.

7. The device of claim 1 , wherein a length of the collection component along the angled fold is about 20 mm or less.

8. The device of claim 1 , wherein the exterior edge of the angled fold forms an acute angle.

9. The device of claim 1 , wherein the active collection surface area or a punched out portion thereof can fit within an amplification reaction volume of about 2 ul to about 100 ul.

10. A kit comprising the device of claim 1 , and optionally, instructions for its use.

11. The kit of claim 10 , further comprising reagents for stabilizing the biological sample for archiving or shipping.

12. The kit of claim 10 , further comprising reagents for amplification of the at least one nucleic acid of the biological sample.

13. The kit of claim 10 , further comprising at least one enclosure to protect the collection component from contamination while archiving or shipping.

14. The kit of claim 10 , wherein the collection component, the handling component, or both comprise an identifier to associate the sample with the substrate from which the sample was collected, wherein the identifier is alphanumeric, graphical, magnetic, or electromagnetic.

15. A system comprising:
a. at least a first imaging component comprising a scanning surface configured to permit an energy wave to penetrate the scanning surface, wherein the energy wave is configured to image the at least one ridge and valley signature of an appendage of the individual; and
b. a substrate having at least a first surface area, wherein the substrate is configured to: a) permit the energy wave to penetrate the substrate to image the appendage in contact with the substrate; and b) collect the biological sample from the appendage; wherein the system is configured to collect at least one ridge and valley signature by imaging the appendage through the scanning surface and the substrate while the appendage is positioned upon the substrate; and
c. the device of claim 1 .

16. The system of claim 15 , further comprising a processor configured to transmit the ridge and valley signature of the individual to at least one database comprising ridge and valley signatures selected from the group consisting of a forensic, criminal, identity, child identity, missing persons, immigration, department of motor vehicles, terrorist, paternity, arrestee, convict database, access control and any combination thereof.

17. A method comprising:
d. providing at least a first imaging component configured to provide an energy wave and comprising a scanning surface configured to permit the energy wave to penetrate the scanning surface;
e. providing a substrate configured to collect a biological sample from an appendage of the individual and configured to permit the energy wave to penetrate the scanning surface, wherein the substrate is positioned over the scanning surface;
f. positioning the appendage of the individual upon the substrate, thereby depositing the biological sample upon the substrate;
g. collecting the at least one ridge and valley signature from the appendage imaged by the energy wave; and
h. collecting the biological sample from the substrate using the device of claim 1 .

18. The method of claim 17 , wherein the substrate or the sample concentration device is prewetted with ethanol, water, or acetonitrile when concentrating the biological sample from the substrate to the sample concentration device.

19. The method of claim 17 , further comprising the step of providing an identifier to associate the biological sample of the individual with the at least one ridge and valley signature of the individual, wherein the identifier is alphanumeric, graphical, magnetic, or electromagnetic.

20. The method of claim 17 , further comprising the step of shipping the substrate containing the biological sample to another location for archiving or testing.

21. The method of claim 17 , further comprising the step of transmitting the at least one ridge and valley signature of the individual to at least one database comprising ridge and valley signatures selected from the group consisting of a forensic, criminal, identity, child identity, missing persons, immigration, department of motor vehicles, terrorist, paternity, arrestee, convict database, access control or any combination thereof.

22. The method of comprising subjecting the biological sample to an analysis, wherein the analysis is at least one of a polymerase chain reaction, a DNA sequencing reaction, STR analysis, SNP analysis, or Indel analysis.

23. The method of claim 22 , further comprising the step of transmitting the identification of the individual provided by the analysis to at least one of a law enforcement agency, immigration control agency, forensics investigative agency, access control agency, licensing agency, or financial services agency.

Assignees

• Life Technologies Corp

Inventors

• Liu Jason Yingjie
• Harrold Michael
• Zhong Chang
• Patel Chandani

Classifications

• A61B10/0045 Primary

  Devices for taking samples of body liquids · CPC title

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• G01N1/30

  Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis} · CPC title

• A61B10/02

  Instruments for taking cell samples or for biopsy {(A61B10/0038 and A61B10/0045 take precedence; needle locating or guiding means A61B17/3403; samplers for enzymology or microbiology C12M1/26; sampling or preparing biological specimens G01N33/48)} · CPC title