Recombinant expression vector applicable to rapid screening for recombinant strain and application
US-12037632-B2 · Jul 16, 2024 · US
Stable, functional chimeric cellobiohydrolase class I enzymes
US9708633B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9708633-B2 |
| Application number | US-201615068521-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 11, 2016 |
| Priority date | Jun 1, 2010 |
| Publication date | Jul 18, 2017 |
| Grant date | Jul 18, 2017 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
First claim
Opening claim text (preview).
What is claimed is: 1. An enzymatic preparation comprising at least two recombinant enzymes comprising at least 98% amino acid sequence identity to a polypeptide having the sequences selected from the group consisting of SEQ ID NO: 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, and 34, wherein each of the enzymes has cellobiohydrolase activity. 2. The enzymatic preparation of claim 1 , wherein the enzymatic preparation comprises a polypeptide having at least 98% sequence identity to the polypeptide of SEQ ID NO: 20 and having cellobiohydrolase activity. 3. The enzymatic preparation of claim 1 , wherein the enzymatic preparation comprises a polypeptide having at least 98% sequence identity to the polypeptide of SEQ ID NO: 34 and having cellobiohydrolase activity. 4. The enzymatic preparation of claim 1 , wherein the enzymatic preparation comprises a polypeptide having at least 98% sequence identity to the polypeptide of SEQ ID NO: 20 and having cellobiohydrolase activity and a polypeptide having at least 98% sequence identity to the polypeptide of SEQ ID NO: 34 and having cellobiohydrolase activity. 5. The enzymatic preparation of claim 1 , wherein the preparation comprises a disrupted cell or microbial preparation. 6. The enzymatic preparation of claim 1 , further comprising one or more enzymes selected from the group consisting of a cellobiohydrolase II, an endoglucanase and a β-glucosidase. 7. A method of treating a biomass comprising cellulose, the method comprising contacting the biomass with the enzymatic preparation of claim 1 . 8. The method of claim 7 , wherein the method is carried out at about 65° C.
Assignees
Inventors
Classifications
Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase · CPC title
Cellulose 1,4-beta-cellobiosidase (3.2.1.91) · CPC title
- C12N9/2437Primary
Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150) · CPC title
- C12Y302/01074Primary
Glucan 1,4-beta-glucosidase (3.2.1.74) · CPC title
- C12P19/14Primary
produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9708633B2 cover?
- The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
- Who is the assignee on this patent?
- The California Inst Of Tech, California Inst Of Techn
- What technology area does this patent fall under?
- Primary CPC classification C12N9/2437. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 18 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).