Culture flask

Patent metadata

Field	Value
Publication number	US-9708576-B2
Application number	US-201314083048-A
Country	US
Kind code	B2
Filing date	Nov 18, 2013
Priority date	Jan 18, 2013
Publication date	Jul 18, 2017
Grant date	Jul 18, 2017

Abstract

A culture flask comprising a culture chamber in a flask body comprising an even bottom wall with a growth surface on the top side, an even cover wall at a section from the bottom wall, and side walls that bridge the distance between the margins of the bottom wall and cover wall, an opening that is in adjacent regions of a side wall and the even cover wall at a distance from the bottom wall, and a hollow cylindrical flask neck that is connected to the margin of the opening and is aligned at a sharp angle to the growth surface so that the serological pipette, a scraper, a cannula or another elongated device for adding or removing material can be placed on the rear corners of the growth surface from the outside through the flask neck.

First claim

What is claimed is:

1. A culture flask comprising:
a culture chamber in a flask body having an even bottom wall with a growth surface on the top side, an even cover wall at a section from the bottom wall, and side walls that bridge the distance between the bottom wall and even cover wall;
an opening in adjacent regions of a side wall and of the even cover wall at a distance from the bottom wall;
a hollow cylindrical flask neck that is connected to the opening and is aligned at a sharp angle to the growth surface so that a serological pipette, a scraper, a cannula or another elongated device for adding or removing material can be placed on the rear corners of the growth surface from the outside through the flask neck;
further comprising a flask base and a flask bonnet, wherein the flask base comprises the bottom wall, the side walls, and a first flask neck part connected to a side wall, the flask bonnet comprises the cover wall and a second flask neck part connected to the cover wall, and the flask base and the flask bonnet are connected to each other in a sealing manner at adjacent margins;
wherein the first flask neck part is a hallow cylinder which, in a bottom margin section of an end face margin that runs in a surface perpendicular to the growth surface, is connected to the margin of a bottom opening section of the opening in the side wall, and wherein the second flask neck part is a hallow cylinder segment that is connected at an end face margin to a top margin section of the end face margin of the first flask neck part, and is connected at the two lateral margins to the margins of a top opening section of the opening in the cover wall that expands toward the first flask neck part;
wherein the connection between the flask base and flask bonnet is an ultrasonic welded connection, and
wherein the ultrasonic welded connection between the flask base and flask bonnet is created by welding an energy director that projects downward and runs around the margin of the flask bonnet into a groove that is open at the top and runs around the top margin of the flask base.

2. The culture flask according to claim 1 , wherein the first flask neck part is a hollow cylinder which, in a bottom margin section of an end face margin that runs in a surface perpendicular to the growth surface, is connected to the margin of a bottom opening section of the opening in the side wall, and wherein the second flask neck part is a hollow cylinder segment that is connected at an end face margin to a top margin section of the end face margin of the first flask neck part, and is connected at the two lateral margins to the margins of a top opening section of the opening in the cover wall that expands toward the first flask neck part.

3. The culture flask according to claim 1 , wherein the flask bonnet has an upwardly projecting, uninterrupted or interrupted peripheral first rib on the margin.

4. The culture flask according to claim 1 , wherein an uninterrupted or interrupted peripheral second rib with an even bottom side projects downward from the margin of the bottom wall.

5. The culture flask according to claim 1 , wherein the flask neck has at least one outer thread and/or at least one screw-in element for a lid.

6. The culture flask according to claim 1 that narrows toward the flask neck.

7. The culture flask according to claim 1 that has a sloping wall angled at a sharp angle to the bottom wall between the bottom wall and side wall to which the flask neck is attached.

8. The culture flask according to claim 1 , wherein the flask neck is a regular cylinder.

9. The culture flask according to claim 1 , wherein the transition from the flask neck and the inside of the culture chamber is completely or partially smooth and/or rounded.

10. The culture flask according to claim 1 with a bonnet and base that are coordinated with each other such that a plurality of equivalent cell culture flasks can be stacked on each other.

11. The culture flask according to claim 1 , wherein the side wall to which the flask neck is attached has, below the flask neck, a cut-out that is suitable for accommodating a region of the flask neck—projecting relative to the cover wall—of an equivalent culture flask on which the culture flask is placed.

12. The culture flask according to claim 1 that is manufactured by injection molding.

13. The culture flask according to claim 1 that is made of polystyrene or another plastic.

14. A culture flask comprising:
a culture chamber in a flask body having an even bottom wall with a growth surface on the top side, an even cover wall at a section from the bottom wall, and side walls that bridge the distance between the bottom wall and even cover wall;
an opening in adjacent regions of a side wall and of the even cover wall at a distance from the bottom wall;
a hallow cylindrical flask neck that is connected to the opening and is aligned at a sharp angle to the growth surface so that a serological pipette, a scraper, a cannula or another elongated device for adding or removing material can be placed on the rear corners of the growth surface from the outside through the flask neck;
further comprising a flask base and a flask bonnet, wherein the flask base comprises the bottom wall, the side walls, and a first flask neck part connected to a side wall, the flask bonnet comprises the cover wall and a second flask neck part connected to the cover wall, and the flask base and flask bonnet are connected to each other in a sealing manner at adjacent margins;
wherein the first flask neck part is hollow cylinder which, in a bottom margin section of an end face margin that runs in a surface perpendicular to the growth surface, is connected to the margin of a bottom opening section of the opening in the side wall, and wherein the second flask neck part is a hollow cylinder segment that is connected at an end face margin to a top margin section of the end face margin of the first flask neck part, and is connected at the two lateral margins to the margins of a top opening section of the opening in the cover wall that expands toward the first flask neck part;
wherein the connection between the flask base and flask bonnet is and infrared welded connection, and
wherein the infrared welded connection between the flask base and flask bonnet is created by melting and pressing together a downward-projecting third rib running around the margin of the flask bonnet, and an upward-projecting fourth rib running around the top margin of the flask base.

Assignees

• Eppendorf Ag

Inventors

• Seippel Martin

Classifications

• C12M33/04 Primary

  by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02) · CPC title

• C12M23/08

  Flask, bottle or test tube · CPC title

• C12M23/02

  Form or structure of the vessel (large containers B65D88/00) · CPC title