Reagent holder, and kits containing same

Patent metadata

Field	Value
Publication number	US-9701957-B2
Application number	US-21841608-A
Country	US
Kind code	B2
Filing date	Jul 14, 2008
Priority date	Jul 13, 2007
Publication date	Jul 11, 2017
Grant date	Jul 11, 2017

Abstract

A holder for reagents, such as may be used for transporting the reagents and for carrying out processing operations on biological samples with the reagents. The holders typically hold reagents for amplifying polynucleotides extracted from the samples. The holder comprises a connecting member; a process tube affixed to the connecting member and having an aperture located in the connecting member; at least one socket, located in the connecting member, and configured to accept a pipette tip; two or more reagent tubes disposed on the underside of the connecting member, each having an inlet aperture located in the connecting member; and one or more receptacles, located in the connecting member and each being configured to receive a reagent tube. Also described are reagent tubes configured with stellated shaped patterns, on their bottom interior surfaces, configured to facilitate complete or near-complete withdrawal of fluid from the tube, via a pipette tip.

First claim

What is claimed is:

1. A unitized reagent holder and reagent system, comprising:
a strip, to which is attached:
a single process tube;
at least three reagent tubes;
a sample lysis reagent in a first said reagent tube;
a PCR reagent for a first analyte in a second said reagent tube;
one or more liquid reagents in a third said reagent tube; and
two or more sockets holding two or more pipette tips, wherein the ratio of sockets holding pipette tips to process tubes is at least two.

2. The unitized reagent holder of claim 1 , further comprising a laminate that seals closed an inlet of each of the containers.

3. The unitized reagent holder of claim 1 , wherein at least one of the containers is removable.

4. The unitized reagent holder of claim 1 , further comprising a pipette tip sheath configured to surround one of the pipette tips.

5. The unitized reagent holder of claim 1 , further comprising a waste tube attached to the strip.

6. A reagent holder and reagent
system comprising:
a single process tube;
at least three reagent tubes;
a sample lysis reagent in a first said reagent tube;
a PCR reagent in a second said reagent tube;
one or more liquid reagents in a third said reagent tube;
two or more sockets holding pipette tips, wherein the ratio of sockets holding pipette tips to process tubes is at least two; and
wherein the process tube, the two or more sockets configured to hold pipette tips, and the reagent tubes are all joined to a single connecting member.

7. The reagent holder and reagent system of claim 6 , further comprising:
a waste tube; and
a pipette tip sheath configured to surround at least one of the pipette tips.

8. The reagent holder and reagent system of claim 6 , wherein the connecting member has a first end and a second end, and the process tube is located at either of the first or second ends.

9. The unitized reagent holder of claim 1 , wherein one of the containers holds a release solution.

Assignees

• Wilson Kerry
• Handique Kalyan
• Brahmasandra Sundaresh N
• Williams Jeff
• Handylab Inc

Inventors

• Wilson Kerry
• Handique Kalyan
• Brahmasandra Sundaresh N
• Williams Jeff

Classifications

• C12N15/1013 Primary

  by using magnetic beads · CPC title

• C12Q1/6813

  Hybridisation assays · CPC title

• C07H1/08

  from natural products · CPC title

• C07C233/36

  having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton · CPC title

• C08G83/003

  Dendrimers · CPC title