Bradyrhizobium strains

Patent metadata

Field	Value
Publication number	US-9700057-B2
Application number	US-201414540501-A
Country	US
Kind code	B2
Filing date	Nov 13, 2014
Priority date	Dec 16, 2011
Publication date	Jul 11, 2017
Grant date	Jul 11, 2017

Abstract

According to the present invention new isolates of Bradyrhizobium japonicum have been isolated and possess unique properties. These Bradyrhizobia are plant growth-promoting rhizobacterium (PGPR), possess superior tolerance/resistance to desiccation, and enhance the overall performance of leguminous plant growth.

First claim

The invention claimed is:

1. A method of treating seeds comprising applying to said seeds an inoculum that comprises at least one mutagenized Bradyrhizobium japonicum strain selected from the group consisting of:
the strain having the deposit accession number NRRL B-50612;
the strain having the deposit accession number NRRL B-50611;
the strain having the deposit accession number NRRL B-50610;
the strain having the deposit accession number NRRL B-50609; and
the strain having the deposit accession number NRRL B-50608.

2. The method of claim 1 , wherein said inoculum further comprises one or more plant signal molecules.

3. The method of claim 1 , wherein said inoculum further comprises a lipo-chitooligosaccharide (LCO).

4. The method of claim 3 , wherein said LCO is synthetic.

5. The method of claim 3 , wherein said LCO is recombinant.

6. The method of claim 3 , wherein said LCO is naturally occurring.

7. The method of claim 3 , wherein said LCO is obtained from a species of Rhizobia selected from the group consisting of Rhizobium spp., Sinorhizobium spp., and Azorhizobium spp.

8. The method of claim 3 , wherein said LCO is obtained from Bradyrhizobium japonicum.

9. The method of claim 3 , wherein said LCO is obtained from an arbuscular mycorrhizal fungus.

10. The method of claim 1 , wherein said inoculum further comprises a chitinous compound.

11. The method of claim 10 , wherein said chitinous compound is a chito-oligomer (CO).

12. The method of claim 11 , wherein said CO is synthetic.

13. The method of claim 11 , wherein said CO is recombinant.

14. The method of claim 11 , wherein said CO is naturally occurring.

15. The method of claim 1 , wherein said inoculum further comprises a flavonoid.

16. The method of claim 15 , wherein said flavonoid is selected from the group consisting of luteolin, apigenin, tangeritin, quercetin, kaempferol, myricetin, fisetin, isorhamnetin, pachypodol, rhamnazin, hesperetin, naringenin, formononetin, eriodictyol, homoeriodictyol, taxifolin, dihydroquercetin, dihydrokaempferol, genistein, daidzein, glycitein, catechin, gallocatechin, catechin 3-gallate, gallocatechin 3-gallate, epicatechin, epigallocatechin, epicatechin 3-gallate, epigallocatechin 3-gallate, cyanidin, delphinidin, malvidin, pelargonidin, peonidin, petunidin, and derivatives thereof.

17. The method of claim 1 , wherein said inoculum further comprises jasmonic acid or a derivative thereof.

18. The method of claim 1 , wherein said inoculum further comprises linoleic acid or a derivative thereof.

19. The method of claim 1 , wherein said inoculum further comprises linolenic acid or a derivative thereof.

20. The method of claim 1 , wherein said inoculum further comprises a karrikin.

21. The method of claim 1 , wherein said inoculum further comprises a herbicide, an insecticide and/or a fungicide.

22. The method of claim 1 , wherein said inoculum further comprises at least one phosphate-solubilizing microorganism.

23. The method of claim 22 , wherein said at least one phosphate-solubilizing microorganism comprises a strain of Penicillium.

24. The method of claim 22 , wherein said at least one phosphate-solubilizing microorganism comprises a strain of P. bilaiae.

25. The method of claim 24 , wherein said strain of P. bilaiae is selected from the group consisting of the strain having the deposit accession number NRRL 50162, the strain having the deposit accession number NRRL 50169, the strain having the deposit accession number ATCC 20851, the strain having the deposit accession number ATCC 22348, and the strain having the deposit accession number ATCC 18309.

26. The method of claim 22 , wherein said at least one phosphate-solubilizing microorganism comprises a strain of P. gaestrivorus.

27. The method of claim 26 , wherein said strain of P. gaestrivorus is the strain having the deposit accession number NRRL 50170.

28. The method of claim 1 , further comprising the step of storing treated seeds in a substantially moisture free environment for a period of at least 1 month, at least 2 months, at least 3 months, at least 4 months, at least 5 months, at least 6 months, at least 1 year or more.

29. The method of claim 1 , wherein said seeds are leguminous plant seeds.

30. The method of claim 29 , wherein said leguminous plant seeds are soybean seeds.

Assignees

• Novozymes Bioag As

Inventors

• Kang Yaowei
• Tran Anh
• Semones Shawn

Classifications

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• A01H3/00

  Processes for modifying phenotypes {, e.g. symbiosis with bacteria} (A01H4/00 takes precedence) · CPC title

• C12R1/01

  Chemistry & Metallurgy · mapped topic

• A01N63/00 Primary

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title

• A01N63/10 Primary

  Animals; Substances produced thereby or obtained therefrom · CPC title