Ion fragmentation

Patent metadata

Field	Value
Publication number	US-9697997-B2
Application number	US-201313840739-A
Country	US
Kind code	B2
Filing date	Mar 15, 2013
Priority date	Feb 14, 2013
Publication date	Jul 4, 2017
Grant date	Jul 4, 2017

Abstract

A collision cell for a mass spectrometer arranged to receive ions for fragmentation in a chamber and comprising an activation ion generator configured to irradiate the received ions with activation ions of the same polarity as the received ions. The activation ion generator is preferably a plasma generator, configured to generate a plasma comprising the activation ions.

First claim

The invention claimed is:

1. A collision cell arranged to receive and trap ions for fragmentation in a chamber and comprising an activation ion generator configured to irradiate the received and trapped ions with activation ions of the same polarity as the received and trapped ions, wherein the activation ion generator is a plasma generator, configured to generate a plasma comprising the activation ions having an energy when they irradiate the received ions of between 700 eV and 2 keV.

2. The collision cell of claim 1 , wherein the activation ion generator is configured to irradiate the trapped ions with a charged gas comprising the activation ions of the same polarity as the received ions.

3. The collision cell of claim 1 , wherein the plasma generator comprises a plasma chamber, arranged to receive a gas and to generate the plasma comprising the activation ions using the received gas.

4. The collision cell of claim 3 , wherein the plasma generator further comprises a microwave energy generator configured to irradiate gas received in the plasma chamber and generate the plasma thereby.

5. The collision cell of claim 3 , further comprising an excitation field generator arranged to excite the plasma generated within the plasma chamber and increase plasma density thereby.

6. The collision cell of claim 5 , wherein the excitation field generator comprises a magnetic field generator, configured to impose the effect of a cyclotron resonance on the generated plasma.

7. The collision cell of claim 3 , wherein the plasma generator further comprises extraction ion optics configured to transfer the activation ions from the plasma chamber towards the collision cell chamber.

8. The collision cell of claim 7 , wherein the extraction ion optics comprises:
a first grid electrode;
a second grid electrode, spaced apart from the first grid electrode; and
a power supply arrangement configured to provide a first potential to the first grid electrode and a second potential to the second grid electrode, the first and second potentials being of opposite polarity.

9. The collision cell of claim 1 , further comprising:
an interface between the activation ion generator and the collision cell chamber; and
a pumping arrangement configured to provide a vacuum in the interface.

10. The collision cell of claim 9 , wherein the interface further comprises an ion optics lens, configured to facilitate transfer of the activation ions from the activation ion generator to the collision cell chamber.

11. The collision cell of claim 1 , further comprising trapping electrodes configured to provide a trapping field for confinement of the received ions to the chamber.

12. The collision cell of claim 11 , wherein the trapping electrodes are configured to receive one or more DC potentials in order to generate the trapping field.

13. The collision cell of claim 12 , further comprising a controller, configured to control the DC potential applied to the trapping electrodes such that:
a first set of DC potentials is applied to the trapping electrodes during a first time period, in order to cause ions for fragmentation to enter the collision cell chamber; and
a second set of DC potentials is applied to the trapping electrodes during a second time period, subsequent to the first time period, the first and second potentials having opposite polarities.

14. The collision cell of claim 13 , wherein the controller is further configured to control the DC potential applied to the trapping electrodes such that a third set of DC potentials is applied to the trapping electrodes during a third time period, in order to cause fragmented ions to exit the collision cell chamber.

15. The collision cell of claim 14 , wherein the first set of DC potentials is set such that ions enter the collision cell chamber in a first direction and wherein the third set of DC potentials is set such that ions exit the collision cell chamber in a second direction, opposite to the first direction.

16. The collision cell of claim 1 , wherein the chamber comprises an ion receiving aperture, configured to allow entrance of ions into the chamber for fragmentation.

17. The collision cell of claim 16 , wherein the ion receiving aperture is also configured to allow exit of fragment ions from the chamber.

18. The collision cell of claim 1 , wherein the energy of the activation ions when they irradiate the trapped ions is between 1 keV and 2 keV.

19. The collision cell of claim 1 , wherein the energy of the activation ions when they irradiate the trapped ions is between 700 eV and 1500 eV.

20. The method of claim 1 , wherein the charge of the trapped ions increases when irradiated by the activation ions.

21. A mass spectrometer, comprising:
an ion source for generating ions;
a collision cell, arranged to receive and trap generated ions and to fragment the trapped ions, the collision cell comprising an activation ion generator configured to irradiate the trapped ions with activation ions of the same polarity as the trapped ions, wherein the activation ion generator is a plasma generator, configured to generate a plasma comprising the activation ions having an energy of between 700 eV and 2 keV when they irradiate the trapped ions; and
a mass analyser, configured to receive fragment ions for analysis.

22. The mass spectrometer of claim 21 , wherein the energy of the activation ions when they irradiate the trapped ions is between 1 keV and 2 keV.

23. The mass spectrometer of claim 21 , wherein the energy of the activation ions when they irradiate the trapped ions is between 700 eV and 1500 eV.

24. The method of claim 21 , wherein the charge of the trapped ions increases when irradiated by the activation ions.

25. A method of ion fragmentation, comprising:
receiving and trapping ions for fragmentation in a collision cell chamber; and
receiving a gas in a plasma chamber of a plasma generator;
generating the plasma comprising the activation ions from the gas in the plasma chamber;
irradiating the trapped ions with activation ions of the same polarity as the trapped ions, wherein the energy of the activation ions when they irradiate the trapped ions is between 700 eV and 2 keV.

26. The method of claim 25 , wherein the step of generating further comprises generating the plasma comprising the activation ions by irradiating the gas received in the plasma chamber with microwave energy.

27. The method of claim 25 , wherein the step of irradiating further comprises exciting the plasma generated within the plasma chamber, in order to increase plasma density thereby.

28. The method of claim 27 , wherein the step of exciting comprises imposing the effect of a cyclotron resonance on the generated plasma using a magnetic field.

29. The method of claim 25 , wherein the step of irradiating further comprises transferring the activation ions from the plasma chamber towards the collision cell chamber using extraction ion optics.

30. The method of claim 29 , wherein the step of transferring comprises:
providing a first potential to a first grid electrode of the extraction ion optics;
providing a second potential to a second grid electrode of the extraction ion optics, spaced apart from the first grid electrode; and
wherein the first and second potentials are of opposite polarity.

31. The method of

Assignees

• Chingin Konstantin
• Zubarev Roman
• Thermo Fisher Scient (Bremen) Gmbh

Inventors

• Chingin Konstantin
• Zubarev Roman

Classifications

• H01J49/0095

  Particular arrangements for generating, introducing or analyzing both positive and negative analyte ions (ion/ion reactions H01J49/0072) · CPC title

• H01J49/0072 Primary

  by ion/ion reaction, e.g. electron transfer dissociation, proton transfer dissociation · CPC title

• H01J49/14

  using particle bombardment, e.g. ionisation chambers · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• H01J49/005

  by collision with gas, e.g. by introducing gas or by accelerating ions with an electric field · CPC title