Promoter variants for expressing genes in a fungal cell

Patent metadata

Field	Value
Publication number	US-9695429-B2
Application number	US-201514850448-A
Country	US
Kind code	B2
Filing date	Sep 10, 2015
Priority date	Nov 18, 2002
Publication date	Jul 4, 2017
Grant date	Jul 4, 2017

Abstract

The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

First claim

What is claimed is:

1. An isolated promoter variant comprising SEQ ID NO: 3.

2. The promoter variant of claim 1 , which increases expression of a nucleic acid sequence encoding a polypeptide compared to the parent promoter of SEQ ID NO: 1.

3. A nucleic acid construct comprising a nucleic acid sequence encoding a polypeptide operably linked to the promoter variant of claim 1 .

4. A recombinant expression vector comprising the nucleic acid construct of claim 3 .

5. A recombinant host cell comprising the nucleic acid construct of claim 3 .

6. A method for producing a polypeptide, comprising (a) cultivating a homologously recombinant cell, having incorporated therein an introduced transcription unit comprising the promoter variant of claim 1 , an exon, and/or a splice donor site operably linked to a second exon of an endogenous nucleic acid sequence encoding the polypeptide, under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.

7. A method for producing a polypeptide, comprising:
(a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising the promoter variant of claim 1 ; and
(b) isolating the polypeptide from the cultivation medium.

8. The method of claim 7 , wherein the promoter variant comprising SEQ ID NO: 3 increases expression of the first nucleic acid sequence compared to the parent promoter of SEQ ID NO: 1.

9. The method of claim 7 , wherein the fungal host cell contains one or more copies of the first nucleic acid sequence.

10. The method of claim 7 , wherein the fungal host cell contains one copy of the first nucleic acid sequence.

11. The method of claim 7 , wherein the polypeptide is selected from the group consisting of an antigen, enzyme, growth factor, hormone, immunodilator, neurotransmitter, receptor, reporter protein, structural protein, and transcription factor.

12. The method of claim 7 , wherein the polypeptide is native or foreign to the fungal host cell.

Assignees

• Novozymes Inc

Inventors

• Yaver Debbie
• Nham Peter

Classifications

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• C12N15/80 Primary

  for fungi · CPC title

• C12P21/00

  Preparation of peptides or proteins (single cell protein C12N1/00) · CPC title

• C12P1/02

  by using fungi · CPC title

• C12N9/0044

  acting on other nitrogen compounds as donors (1.7) · CPC title