Acoustic waves in microfluidics

Patent metadata

Field	Value
Publication number	US-9695390-B2
Application number	US-201113818146-A
Country	US
Kind code	B2
Filing date	Aug 23, 2011
Priority date	Aug 23, 2010
Publication date	Jul 4, 2017
Grant date	Jul 4, 2017

Abstract

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.

First claim

What is claimed is:

1. An article, comprising:
a microfluidic substrate having defined therein a microfluidic system containing an inlet microfluidic channel and more than two outlet channels meeting at a single junction;
a tapered interdigitated transducer positioned on a material; and
at least two coupling regions, each of which is positioned to refract surface acoustic waves generated by the tapered interdigitated transducer towards at least a portion of the single junction, wherein each coupling region physically connects the material and the microfluidic substrate.

2. The article of claim 1 , comprising at least five outlet channels meeting at the single junction with the inlet microfluidic channel.

3. The article of claim 1 , further comprising a surface acoustic wave generator comprising the interdigitated transducer.

4. The article of claim 1 , wherein the interdigitated transducer has a finger spacing of between about 20 micrometers and about 30 micrometers.

5. The article of claim 4 , wherein the interdigitated transducer comprises a first electrode and a second electrode that are interdigitated with each other.

6. The article of claim 1 , wherein the material is a piezoelectric substrate.

7. The article of claim 6 , wherein the piezoelectric substrate comprises LiNbO 3 .

8. The article of claim 1 , wherein the microfluidic substrate comprises polydimethylsiloxane.

Assignees

• Weitz David A
• Franke Thomas
• Wixforth Achim
• Schmid Lothar
• Agresti Jeremy
• Abate Adam R
• Harvard College
• Universität Augsburg

Inventors

• Weitz David A
• Franke Thomas
• Wixforth Achim
• Schmid Lothar
• Agresti Jeremy
• Abate Adam R

Classifications

• G01N2015/1006

  for cytology · CPC title

• Y10T137/0391

  Affecting flow by the addition of material or energy · CPC title

• B01L2400/0421

  electrophoretic flow · CPC title

• B01L2200/0636

  Focussing flows, e.g. to laminate flows · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title