Peptide domains that bind small molecules

Patent metadata

Field	Value
Publication number	US-9695217-B2
Application number	US-201514968386-A
Country	US
Kind code	B2
Filing date	Dec 14, 2015
Priority date	Jan 29, 2010
Publication date	Jul 4, 2017
Grant date	Jul 4, 2017

Abstract

Described herein are small peptide domains and consensus sequences that bind small target molecules of industrial importance, e.g., metals such as nickel, β carotene, and isoflavones such as genistein. Also described are fusion proteins containing such binding domains fused to proteins or to peptide domains like GST or CBD that bind other ligands and can be used to immobilize the target binding domain on a support. One class of fusion proteins that is useful in industrial settings are fusions that contain concatemers of target binding domains, which increases the binding equivalents per molecule.

First claim

What is claimed is:

1. An isolated non-naturally occurring fusion polypeptide or isolated non-naturally occurring fusion peptide comprising a carotenoid binding peptide comprising a sequence of the formula
X 1 X 2 GWX 3 HyX 4 X 5 X 6   (SEQ ID NO:120)

Wherein Hy is an aromatic amino acid; X 1 is alanine, valine, leucine, glutamine, tryptophan, tyrosine, serine, proline, threonine or isoleucine; X 2 is alanine, glycine, isoleucine or valine; X 3 is tryptophan, methionine, glycine, proline, leucine, or serine; X 4 is tryptophan, phenylalanine, methionine, glycine, threonine, or histidine; X 5 is glycine, tryptophan, serine, phenylalanine, leucine, glutamine, or alanine; and X 6 is threonine, glycine, tryptophan, alanine, methionine, asparagine, or valine.

2. The isolated polypeptide or peptide of claim 1 wherein X 2 is alanine.

3. The isolated polypeptide or peptide of claim 1 wherein Hy is tryptophan.

4. The isolated polypeptide or peptide of claim 1 wherein X 4 is a tryptophan.

5. An isolated non-naturally occurring fusion polypeptide or isolated non-naturally occurring fusion peptide comprising a carotenoid binding peptide comprising a sequence
X 1 WX 2 Hy  (SEQ ID NO:121)

where Hy is an aromatic amino acid; X 2 is any amino acid; and X 1 is a single amino acid selected from glycine, proline or leucine.

6. The isolated polypeptide or peptide of claim 5 wherein X 1 is glycine.

Assignees

• Univ Iowa State Res Found Inc
• Archer-Daniels-Midland Company

Inventors

• Binder Thomas P
• Rao Aragula Gururaj
• Yamamoto Yasufumi
• Hanke Paul D

Classifications

• C07K14/00

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• C07K5/08

  Tripeptides · CPC title

• C07K7/06 Primary

  having 5 to 11 amino acids · CPC title

• G01N33/68

  involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)} · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title