Transformed Synechococcus elongatus having capability of producing acetone from carbon dioxide

Patent metadata

Field	Value
Publication number	US-9688976-B2
Application number	US-201615057551-A
Country	US
Kind code	B2
Filing date	Mar 1, 2016
Priority date	Mar 4, 2015
Publication date	Jun 27, 2017
Grant date	Jun 27, 2017

Abstract

Disclosed herein are a transformed Synechococcus elongatus strain having improved capability of producing acetone and a method for producing acetone and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce acetone with high selectivity using carbon dioxide as a carbon source. The present disclosure is economical because the Synechococcus elongatus strain can economically produce high value-added acetone using carbon dioxide existing in the atmosphere as a carbon source without requiring an additional catalytic reaction. Also, the present disclosure is environment-friendly because carbon dioxide in the atmosphere can be removed or reduced using the microorganism.

First claim

What is claimed is:

1. A Synechococcus elongatus strain being capable of enhanced production of acetone, wherein the strain comprises:
one or more selected from a group consisting of:
an acetyl-CoA transferase gene containing SEQ ID NO: 1 and an acetyl-CoA synthase gene contains SEQ ID NO: 2;
an acetoacetyl-CoA transferase gene containing SEQ ID NO: 3 or SEQ ID NO: 4; and
an acetoacetate decarboxylase gene containing SEQ ID NO: 5,
wherein the strain produces acetone in a molar ratio of 0.8 or greater in the total product under a condition of 30° C. and 5% carbon dioxide.

2. The strain according to claim 1 , wherein the strain is one transformed with a vector comprising: one or more selected from a group consisting of an acetyl-CoA transferase gene containing SEQ ID NO: 1 and an acetyly-CoA synthase gene containing SEQ ID NO: 2;
an acetoacetyl-CoA transferase gene containing SEQ ID NO: 3 or SEQ ID NO: 4; and
an acetoacetate decarboxylase gene containing SEQ ID NO: 5.

3. The strain according to claim 2 , wherein the strain is Synechococcus elongatus PCC7942 transformed with the vector.

4. The strain according to claim 2 , wherein the vector further comprises:
a pUC replication origin as a replication origin;
neutral sites located upstream and downstream of the replication origin;
a spectinomycin resistance gene as a selection marker;
a repressor selected from a group consisting of a lac I repressor, a tetR repressor and an AraC repressor;
a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter; and
a BglII site, a BamHI site, an EcoRI site and an XhoI site as restriction enzyme sites.

5. The strain according to claim 4 , wherein one or more selected from a group consisting of an acetyl-CoA transferase gene containing SEQ ID NO: 1 and an acetyl-CoA synthase gene containing SEQ ID NO: 2; an acetoacetyl-CoA transferase gene containing SEQ ID NO: 3 or SEQ ID NO: 4; and an acetoacetate decarboxylase gene containing SEQ ID NO: 5 are located between the BglII site and the BamHI site.

6. The strain according to claim 2 , wherein the vector comprises a sequence from SEQ ID NOS: 6-9.

7. The strain according to claim 1 , wherein the strain is a Synechococcus elongatus strain of accession number KCTC12758BP, KCTC12759BP, KCTC12760BP or KCTC12761BP.

8. The strain according to claim 1 , wherein the strain absorbs and fixes carbon dioxide.

Assignees

• Korea Inst Sci & Tech

Inventors

• Woo Han Min
• Um Youngsoon
• Chwa Jun Won
• Gong Gyeongtaek

Classifications

• Y02P60/247

  Cross-Sectional Technologies · mapped topic

• C12N9/13

  transferring sulfur containing groups (2.8) · CPC title

• C12N9/88 Primary

  Lyases (4.) · CPC title

• C12Y602/01001

  Acetate-CoA ligase (6.2.1.1) · CPC title

• C12Y208/03009

  Butyrate--acetoacetate CoA-transferase (2.8.3.9) · CPC title