Cell release method

Patent metadata

Field	Value
Publication number	US-9688963-B2
Application number	US-201514981447-A
Country	US
Kind code	B2
Filing date	Dec 28, 2015
Priority date	Jun 28, 2013
Publication date	Jun 27, 2017
Grant date	Jun 27, 2017

Abstract

Adhesive culture cells cultured by using a culture container made of a flexible film can selectively be released from the culture container without causing damage to the cells. A cell release method for releasing, from a culture container, adhesive culture cells cultured adhering to the culture container made of a flexible film includes bringing a surface of the film of the culture container into contact with the adhesive culture cells, applying a pressure to the adhesive culture cells, and releasing the adhesive culture cells from the culture container, wherein the surface of the film faces a surface of the film of the culture container to which the adhesive culture cells adhere.

First claim

The invention claimed is:

1. A cell release method for releasing, from a culture container made of a flexible film, adherent cells that adhere to the culture container during cell culture, the method comprising:
a) bringing a first side of the culture container into contact with the adherent cells that have adhered to a second side of the culture container, by pressing on the exterior of the first side with a Dressing member, thereby applying pressure to a region below the pressing member, wherein the pressing releases the cells from the second side of the culture container, and
b) removing the adherent cells from the culture container.

2. The cell release method according to claim 1 , wherein the pressing member has a flat bottom surface.

3. The cell release method according to claim 1 , wherein the pressing member has a curved bottom surface.

4. The cell release method according to claim 1 , wherein the pressing member is a roller.

5. The cell release method according to claim 1 , further comprising:
a) disposing a region of the culture container to which the adherent cells which are not to be released have adhered above one or more holes of a mounting base on which the culture container is mounted,
b) pressing a region of the culture container including the region disposed above the one or more holes, and selectively releasing the adherent cells in the pressed region without releasing the cells in the region above the holes.

6. The cell release method according to claim 1 , wherein the first and second sides are brought into contact by removing culture medium and air from the culture container, thereby creating a negative pressure in the culture container, and wherein the released cells are removed by adding culture medium to the culture container and removing a suspension of the cells in the culture medium.

Assignees

• Toyo Seikan Group Holdings Ltd

Inventors

• Suenaga Ryo
• Totani Takahiko

Classifications

• C12N5/0662

  Stem cells · CPC title

• C12N5/0696 Primary

  Artificially induced pluripotent stem cells, e.g. iPS · CPC title

• C12M33/18

  Rollers · CPC title

• C12M33/12 Primary

  by pressure · CPC title

• C12N2509/10

  Mechanical dissociation · CPC title