Ultrafiltration for preparing outer membrane vesicles

Patent metadata

Field	Value
Publication number	US-9687789-B2
Application number	US-56447404-A
Country	US
Kind code	B2
Filing date	Jul 15, 2004
Priority date	Jul 15, 2003
Publication date	Jun 27, 2017
Grant date	Jun 27, 2017

Abstract

In place of a step of centrifugation during preparation of outer membrane vesicles (OMVs) from bacteria, the invention utilizes ultrafiltration. This allows much larger amounts of OMV-containing supernatant to be processed in a much shorter time. Thus the invention provides a process for preparing bacterial OMVs, comprising a step of ultrafiltration. The ultrafiltration step is performed on an aqueous suspension of OMVs after they have been prepared from bacteria and the OMVs remain in suspension after the filtration step. The invention is particularly useful for preparing OMVs from Neisseria meningitidis.

First claim

The invention claimed is:

1. A process for preparing bacterial outer membrane vesicles (OMVs), comprising
(a) applying ultrafiltration to crude OMVs to produce a retentate with the OMVs;
(b) applying ultracentrifugation to the OMVs in the retentate to produce a pellet with the OMVs; and
(c) resuspending the OMVs from the pellet.

2. The process of claim 1 , wherein the crude OMVs are an aqueous suspension of OMVs that have been prepared from bacteria.

3. The process of claim 1 , wherein the ultrafiltration step results in diafiltration.

4. The process of claim 1 , wherein the ultrafiltration is cross-flow or tangential flow.

5. The process of claim 1 , wherein the membrane used for ultrafiltration has a cut-off of about 300 kDa.

6. The process of claim 1 , wherein the OMVs are sterilised after ultrafiltration.

7. The process of claim 6 , wherein the sterilisation is by filter sterilisation.

8. A process for preparing bacterial OMVs, comprising the steps of: (a) cultivating bacterial cells; (b) collecting and/or concentrating the cultivated cells; (c) disrupting the outer membranes of the cultivated cells; and (d) preparing OMVs by the method of claim 1 .

9. The process of claim 1 , further comprising the step of combining the OMVs with a pharmaceutical carrier and/or adjuvants and/or stabiliser.

10. The process of claim 1 , wherein the bacterium for OMV preparation is Gram negative.

11. The process of claim 10 , wherein the bacterium is Neisseria meningitidis.

12. The process of claim 11 , wherein the bacterium is serogroup B N. meningitidis.

13. The process of claim 12 , wherein the bacterium is a B:4:P1.4 strain, a B:4:P1.15 strain, a B:4:P1.19,15 strain, a B:4:P1.7b,4 strain or a B:15:P1.7,16 strain.

14. The process of claim 11 , wherein the N. meningitidis has one or more mutations to decrease or knockout expression of a gene product.

15. The process of claim 14 , wherein the gene product is Cps, CtrA, CtrB, CtrC, CtrD, ExbB, ExbD, FrpB, GalE, HtrB, MsbB, LbpA, LbpB, LpxK, NMB0033, OpA, OpC, PhoP, PilC, PmrE, PmrF, PorA, PorB, rmpM, SiaA, SiaB, SiaC, SiaD, SynA, SynB, SynC, TbpA and/or TbpB.

Assignees

• Olivieri Roberto
• Sabbatini Fabio
• Marsili Ilio
• Glaxosmithkline Biologicals Sa

Inventors

• Olivieri Roberto
• Sabbatini Fabio
• Marsili Ilio

Classifications

• C07K14/22

  from Neisseriaceae (F) · CPC title

• C12N1/005

  after treatment of microbial biomass not covered by C12N1/02 - C12N1/08 · CPC title

• B01D2311/04

  Specific process operations in the feed stream; Feed pretreatment · CPC title

• B01D61/145 Primary

  Ultrafiltration · CPC title

• B01D61/16

  Feed pretreatment · CPC title