Heparosan polymers and methods of making and using same for the enhancement of therapeutics

Patent metadata

Field	Value
Publication number	US-9687559-B2
Application number	US-55632409-A
Country	US
Kind code	B2
Filing date	Sep 9, 2009
Priority date	Mar 19, 2008
Publication date	Jun 27, 2017
Grant date	Jun 27, 2017

Abstract

The present invention includes compositions, methods, and systems for the development and use of heparosan, a natural polymer related to heparin, as a new therapeutic modifying agent or vehicle which can modulate drug cargo pharmacokinetics and behavior within a mammalian patient.

First claim

What is claimed is:

1. A method for preparing a pharmaceutically active conjugate for administration to a mammalian patient, comprising the steps of:
reacting at least one therapeutic drug with at least one heparosan polymer under conditions sufficient to effect covalent conjugation of the at least one therapeutic drug and the at least one heparosan polymer to form a reaction mixture containing one or more drug-heparosan polymer conjugates, wherein the at least one therapeutic drug remains active after conjugation and does not increase immunoreactivity of the at least one heparosan polymer, and wherein the at least one therapeutic drug is an agent used in the treatment of cancer or an inflammatory condition; and
forming a sterile pharmaceutical formulation comprising the drug-heparosan polymer conjugates in a unit dosage format for therapeutic injection into the patient.

2. The method of claim 1 , wherein the one or more drug-heparosan polymer conjugates comprise a therapeutic drug having a single heparosan polymer conjugated thereto.

3. The method of claim 1 , wherein the at least one heparosan polymer has a mass in a range of from about 600 Da to 800 kDa.

4. The method of claim 1 , wherein the at least one heparosan polymer is monodisperse.

5. The method of claim 1 , wherein the at least one heparosan polymer is unsulfated and unepimerized.

6. The method of claim 1 , wherein the drug-heparosan conjugate exhibits increased retention in blood circulation of a mammalian patient when compared to drug alone.

7. The method of claim 1 , wherein the drug-heparosan conjugate exhibits reduced occurrence of accumulation in organs of a mammalian patient when compared to drug alone.

8. The method of claim 1 , wherein the one or more drug-heparosan polymer conjugates comprise a therapeutic drug having multiple heparosan polymers conjugated thereto.

9. The method of claim 1 , wherein the at least one heparosan polymer is further characterized as being substantially non-targeting.

10. The method of claim 1 , further comprising the step of modifying the at least one heparosan polymer prior to reacting with the at least one therapeutic drug to provide at least one reactive group on the at least one heparosan polymer with which a group on the at least one therapeutic drug reacts to effect the covalent conjugation of the at least one therapeutic drug to the at least one heparosan polymer.

11. The method of claim 10 , wherein the at least one reactive group is selected from the group consisting of an aldehyde, alkyne, ketone, maleimide, thiol, azide, amino, hydrazide, phosphate, sulfate, nitrate, carbonate, ester, chelator, and combinations thereof.

12. The method of claim 10 , wherein the step of modifying the at least one heparosan polymer is further defined as creating at least one amine-, carbonyl-, and/or sulfhydryl-reactive group on the at least one heparosan polymer.

13. The method of claim 1 , wherein the at least one therapeutic drug is selected from a therapeutic peptide, polypeptide, or protein.

14. The method of claim 1 , wherein the at least one heparosan polymer is further characterized as having a multi-hour half-life within extracellular compartments of the mammalian patient.

15. The method of claim 14 , wherein the at least one heparosan polymer has a half-life of at least 15 hours within extracellular compartments of the mammalian patient.

16. A method for preparing a pharmaceutically active conjugate for administration to a mammalian patient, comprising the steps of:
modifying at least one heparosan polymer to provide at least one reactive group on the at least one heparosan polymer that is available to form a bond to a therapeutic drug;
reacting at least one therapeutic drug with the at least one modified heparosan polymer under conditions sufficient for the at least one reactive group on the at least one modified heparosan polymer to react with a group on the at least one therapeutic drug and thereby effect covalent conjugation of the at least one therapeutic drug and the at least one heparosan polymer, thereby forming a reaction mixture containing one or more drug-heparosan polymer conjugates, wherein the at least one therapeutic drug remains active after conjugation and does not increase immunoreactivity of the at least one heparosan polymer, and wherein the at least one therapeutic drug is an agent used in the treatment of cancer or an inflammatory condition; and
forming a sterile pharmaceutical formulation comprising the drug-heparosan polymer conjugates in a unit dosage format for therapeutic injection into the patient.

17. A method for preparing a pharmaceutically active conjugate for administration to a mammalian patient, comprising the steps of:
reacting at least one therapeutic drug with at least one heparosan polymer under conditions sufficient to effect covalent conjugation of the at least one therapeutic drug and the at least one heparosan polymer, the at least one heparosan polymer having at least one amine-, carbonyl-, and/or sulfhydryl-reactive group that reacts with a group on the at least one therapeutic drug to conjugate the heparosan polymer and the at least one therapeutic drug, thereby forming a reaction mixture containing one or more drug-heparosan polymer conjugates, wherein the at least one therapeutic drug remains active after conjugation and is not an adjuvant, and wherein the at least one therapeutic drug is an agent used in the treatment of cancer or an inflammatory condition; and
forming a sterile pharmaceutical formulation comprising the drug-heparosan polymer conjugates in a unit dosage format for therapeutic injection into the patient.

18. The method of claim 1 , wherein the at least one therapeutic drug is selected from the group consisting of a cytokine, a hormone, an enzyme, an antibody, an antibody fragment, and combinations thereof.

19. The method of claim 16 , wherein the at least one therapeutic drug is selected from the group consisting of a cytokine, a hormone, an enzyme, an antibody, an antibody fragment, and combinations thereof.

20. The method of claim 17 , wherein the at least one therapeutic drug is selected from the group consisting of a cytokine, a hormone, an enzyme, an antibody, an antibody fragment, and combinations thereof.

21. A method for preparing a pharmaceutically active conjugate for administration to a mammalian patient, comprising the step of:
forming a sterile pharmaceutical formulation comprising at least one drug-heparosan polymer conjugate in a unit dosage format for therapeutic injection into the patient, wherein the drug-heparosan polymer conjugate comprises at least one therapeutic drug covalently conjugated to at least one modified heparosan polymer, wherein the at least one heparosan polymer has been modified to provide at least one reactive group on the at least one heparosan polymer that forms a covalent bond with the at least one therapeutic drug, and wherein the at least one therapeutic drug remains active after conjugation and does not increase immunoreactivity of the at least one heparosan polymer, and wherein the at least one therapeutic drug is at least one of an anti-cancer agent or an anti-inflammatory agent.

22. The method of claim 1 , wherein the therapeutic drug-heparosan polymer conjugate exhibits reduced immunogenicity when compared to therapeutic drug alone.

23. The method of claim 1 , wherein the one or more drug-heparosan polymer conjugates comprises a heparosan polymer having

Assignees

• Deangelis Paul L
• Univ Oklahoma

Inventors

• Deangelis Paul L

Classifications

• A61K47/4823 Primary

  Human Necessities · mapped topic

• A61K47/61 Primary

  the organic macromolecular compound being a polysaccharide or a derivative thereof · CPC title