Anti-cancer agent delivery vehicles capable of improved loading

Patent metadata

Field	Value
Publication number	US-9687464-B2
Application number	US-201113634036-A
Country	US
Kind code	B2
Filing date	Mar 11, 2011
Priority date	Mar 11, 2010
Publication date	Jun 27, 2017
Grant date	Jun 27, 2017

Abstract

There is provided a conjugate of a delivery agent containing a chemical moiety and at least one flavonoid. The flavonoid exists in a monomeric form or dimeric form before conjugation and remains in the monomeric form or dimeric form after conjugation. Preferably, the conjugate comprises two flavonoids. The delivery agent is conjugated at the C6 and/or the C8 position of the A ring of the flavonoid. An anti-cancer agent delivery vehicle comprising an anti-cancer agent and the conjugate is also provided.

First claim

We claim:

1. A conjugate comprising:
(a) at least one polymer selected from the group consisting of amine-terminated poly(ethylene glycol) and succinimide-terminated poly(ethylene glycol), and
(b) at least one flavonoid selected from the group consisting of amine-terminated (−)-epigallocatechin gallate, carboxyl-terminated (−)-epigallocatechin gallate, and succinimide-terminated (−)-epigallocatechin gallate (EGCG);

wherein the polymer is conjugated at the C6 and/or C8 of the A ring of the flavonoid; and wherein the flavonoid exists in a monomeric form or dimeric form before conjugation and remains in the monomeric form or dimeric form after conjugation.

2. The conjugate of claim 1 , wherein the polymer comprises carboxyl-terminated poly(ethylene glycol), amine-terminated poly(ethylene glycol), or succinimide-terminated poly(ethylene glycol) and the flavonoid comprises (−)-epigallocatechin gallate (PEG-EGCG).

3. A delivery vehicle comprising a conjugate of claim 1 .

4. An anti-cancer agent delivery vehicle comprising an anti-cancer agent and a conjugate of claim 1 .

5. The anti-cancer agent delivery vehicle of claim 4 , wherein the anti-cancer agent is a protein, a nucleic acid, a small molecule, a drug, a peptide, an antibody, a hormone, an enzyme, a growth factor, a cytokine, single stranded DNA, double stranded DNA, single stranded RNA, double stranded RNA, a short hairpin RNA, an siRNA, an antibiotic, a chemotherapeutic agent or an angiogenesis inhibitor.

6. The anti-cancer agent delivery vehicle of claim 5 , wherein the anti-cancer agent is one selected from the group consisting of herceptin (trastuzumab), TNP470, doxorubicin, cisplatin, paclitaxel, daunorubicin, and a mixture thereof.

7. A pharmaceutical composition comprising the anti-cancer agent delivery vehicle of claim 4 .

8. The pharmaceutical composition of claim 7 , further comprising a pharmaceutically acceptable carrier.

9. The anti-cancer agent delivery vehicle according to claim 4 for use of delivering the anti-cancer agent to a cell in a subject.

10. The anti-cancer agent delivery vehicle of claim 9 , wherein the anti-cancer agent delivery vehicle is formulated for injection, surgical implantation or topical administration.

11. The conjugate of claim 1 , wherein the conjugate is of nanometer dimensions.

12. The conjugate of claim 1 , comprising at least two flavonoids, wherein the flavonoids do not form oligomers with one another and wherein the flavonoids exists in a monomeric form or dimeric form before conjugation to the polymer and remain in the monomeric form or dimeric form after conjugation to the polymer.

Assignees

• Kurisawa Motoichi
• Liang Kun
• Tan Susi
• Chung Joo Eun
• Ying Jackie Y
• Agency Science Tech & Res

Inventors

• Kurisawa Motoichi
• Liang Kun
• Tan Susi
• Chung Joo Eun
• Ying Jackie Y

Classifications

• A61P9/00

  Drugs for disorders of the cardiovascular system · CPC title

• A61K31/704

  attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin {(digitoxin A61K31/7048)} · CPC title

• A61K31/282 Primary

  Platinum compounds · CPC title

• A61K31/353

  3,4-Dihydrobenzopyrans, e.g. chroman, catechin · CPC title

• A61K9/1075

  Microemulsions or submicron emulsions; Preconcentrates or solids thereof; Micelles, e.g. made of phospholipids or block copolymers (A61K9/0026 takes precedence) · CPC title