Combined mass-to-charge ratio and charge state selection in tandem mass spectrometry

Patent metadata

Field	Value
Publication number	US-9685313-B2
Application number	US-51497207-A
Country	US
Kind code	B2
Filing date	Nov 15, 2007
Priority date	Nov 15, 2006
Publication date	Jun 20, 2017
Grant date	Jun 20, 2017

Abstract

A mass spectrometer is disclosed comprising an ion mobility spectrometer and an ion gate. A collision cell is arranged downstream of the ion gate. The operation of the ion mobility spectrometer and the ion gate are synchronised so that only ions having a particular mass to charge ratio and a desired charge state are onwardly transmitted to the collision cell.

First claim

The invention claimed is:

1. A mass spectrometer comprising:
a first mass to charge ratio filter or mass to charge ratio mass analyser arranged and adapted in a first mode of operation to transmit parent or precursor ions having a mass to charge ratio within a first range;
an ion mobility spectrometer or separator;
an ion gate or ion barrier, arranged downstream of the ion mobility spectrometer or separator, for attenuating ions in a mode of operation;
a collision, fragmentation or reaction device; and
a control device configured to control the operation of said ion gate or ion barrier so that ions from the parent or precursor ions having mass to charge ratios within said first range but having one or more undesired first charge states are substantially attenuated by the ion gate or ion barrier.

2. A mass spectrometer as claimed in claim 1 , wherein said first mass to charge ratio filter or mass to charge ratio mass analyser is arranged and adapted in said first mode of operation to attenuate ions having mass to charge ratios outside of said first range.

3. A mass spectrometer as claimed in claim 1 , wherein said ion gate or ion barrier is arranged to transmit ions during a first time period or first time window and to attenuate or divert ions during a second time period or second time window, wherein said second time period or second time window is different from said first time period or first time window.

4. A mass spectrometer as claimed in preceding claim 1 , wherein said first undesired charge state is selected from two or more of the following: (i) singly charged; (ii) doubly charged; (iii) triply charged; (iv) quadruply charged; (v) quintuply; and (vi) multiply charged.

5. A mass spectrometer as claimed in claim 1 , wherein said first mass to charge ratio filter or mass to charge ratio mass analyser is arranged downstream of said ion mobility spectrometer or separator.

6. A method of mass spectrometry using a mass spectrometer having an ion mobility spectrometer or separator and a collision, fragmentation or reaction device comprising:
transmitting parent or precursor ions having mass to charge ratios within a first range through a first mass to charge ratio filter or mass to charge ratio mass analyser; and
attenuating ions from the parent or precursor ions in a mode of operation with an ion gate or ion barrier that is arranged downstream of the ion mobility spectrometer or separator by controlling the operation of said ion gate or ion barrier so that the ions from the parent or precursor ions having mass to charge ratios within said first range but having one or more first undesired charge states are substantially attenuated.

7. A mass spectrometer comprising:
a first mass to charge ratio filter or mass to charge ratio mass analyser arranged and adapted in a first mode of operation to transmit parent or precursor ions having a mass to charge ratio within a first range;
an ion mobility spectrometer or separator;
means for attenuating ions from the parent or precursor ions in a mode of operation, comprising means for removing, attenuating or reducing the amplitude of an AC or RF voltage or potential which is applied to at least a portion of said ion mobility spectrometer or separator;
a collision, fragmentation or reaction device; and
a control device configured to control the operation of said means for attenuating ions so that the ions from the parent or precursor ions having mass to charge ratios within said first range but having one or more undesired first charge states are substantially attenuated.

8. A mass spectrometer as claimed in claim 7 , wherein, in use, said means for attenuating ions removes, attenuates or reduces the amplitude of an AC or RF voltage or potential which is applied to at least a portion of said ion mobility spectrometer or separator so that ions within at least a portion of said ion mobility spectrometer or separator are no longer confined radially within said ion mobility spectrometer or separator.

9. A mass spectrometer as claimed in claim 7 , wherein said first mass to charge ratio filter or mass to charge ratio mass analyser is arranged and adapted in said first mode of operation to attenuate the ions from the parent or precursor ions having mass to charge ratios outside of said first range.

10. A mass spectrometer as claimed in claim 7 , wherein said means for attenuating ions is arranged to transmit ions during a first time period or first time window and wherein said attenuation means is arranged to attenuate or divert ions during a second time period or second time window, wherein said second time period or second time window is different from said first time period or first time window.

11. A mass spectrometer as claimed in claim 7 , wherein said first undesired charge state is selected from two or more of the following: (i) singly charged; (ii) doubly charged; (iii) triply charged; (iv) quadruply charged; (v) quintuply; and (vi) multiply charged.

12. A mass spectrometer as claimed in claim 7 , wherein said first mass to charge ratio filter or mass to charge ratio mass analyser is arranged downstream of said ion mobility spectrometer or separator.

13. A method of mass spectrometry using a mass spectrometer having an ion mobility spectrometer or separator and a collision, fragmentation or reaction device comprising:
transmitting parent or precursor ions having mass to charge ratios within a first range through a first mass to charge ratio filter or mass to charge ratio mass analyser; and
attenuating ions in a mode of operation by removing, attenuating or reducing the amplitude of an AC or RF voltage or potential which is applied to at least a portion of said ion mobility spectrometer or separator so that ions from the parent or precursor ions having mass to charge ratios within said first range but having one or more first undesired charge states are substantially attenuated.

14. A method of mass spectrometry as claimed in claim 13 , wherein removing, attenuating or reducing the amplitude of an AC or RF voltage or potential which is applied to at least a portion of said ion mobility spectrometer or separator is performed so that ions within at least a portion of said ion mobility spectrometer or separator are no longer confined radially within said ion mobility spectrometer or separator.

15. A mass spectrometer comprising:
a first mass to charge ratio filter or mass to charge ratio mass analyser arranged and adapted in a first mode of operation to transmit parent or precursor ions having a mass to charge ratio within a first range;
an ion mobility spectrometer or separator;
means for attenuating ions in a mode of operation, comprising means for removing, attenuating or reducing the amplitude of an AC or RF voltage or potential which is applied to at least a portion of an ion guide or other ion optical device arranged downstream of said ion mobility spectrometer or separator;
a collision, fragmentation or reaction device; and
a control device configured to control the operation of said means for attenuating ions so that ions from the parent or precursor ions having mass to charge ratios within said first range but having one or more undesired first charge states are substantially attenuated.

16. A mass spectrometer as claimed in claim 15 , wherein when, in use, said means for attenuating ions removes, attenuates or reduces the amplitude of an AC or RF voltage or potential which is applied to at least a portion of said ion guide or other ion optical device which is arranged downstream of said ion mobility spectrometer or separator, then ions wit

Assignees

• Bateman Robert Harold
• Giles Kevin
• Pringle Steven Derek
• Wildgoose Jason Lee
• Micromass Ltd

Inventors

• Bateman Robert Harold
• Giles Kevin
• Pringle Steven Derek
• Wildgoose Jason Lee

Classifications

• H01J49/004 Primary

  Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn · CPC title

• H01J49/4215 Primary

  Quadrupole mass filters (H01J49/4225 takes precedence) · CPC title

• H01J49/426

  Methods for controlling ions · CPC title

• G01N27/622

  Ion mobility spectrometry · CPC title

• H01J49/26

  Mass spectrometers or separator tubes · CPC title