Detection device, detection kit, and detection method

Patent metadata

Field	Value
Publication number	US-9684003-B2
Application number	US-201615244468-A
Country	US
Kind code	B2
Filing date	Aug 23, 2016
Priority date	Oct 28, 2015
Publication date	Jun 20, 2017
Grant date	Jun 20, 2017

Abstract

Disclosed is a detection method, including steps of: providing an elongated carrier with a plurality of recognition substances fixed thereon; bringing a first end of the elongated carrier in contact with a liquid sample to permit the liquid sample to move towards an opposite second end along the elongated carrier, therewith a plurality of coloring elements in the liquid sample forming a colored band on the elongated carrier; and determining whether the liquid sample contains analytes based on the length of the colored band. Accordingly, the analytes can be detected by recognizing the agglutination based on the length of the colored band. Additionally, the present invention also provides a detection device and a detection kit for the aforementioned detection process.

First claim

What is claimed is:

1. A detection device, comprising:
an elongated carrier having a spiral segment with a plurality of loops along a central axis, and having a first end and an opposite second end, wherein the elongated carrier allows a liquid sample to move from the first end toward the second end and reach the spiral segment after the first end contacts with the liquid sample, wherein an agglutination colored band or an expanded colored band extending from the first end toward the second end of the elongated carrier is formed by a plurality of coloring elements in the liquid sample; and
a plurality of recognizing substances being fixed on the elongated carrier, the recognizing substances being able to bind to a plurality of analytes in the liquid sample so that the coloring elements form the agglutination colored band, wherein a length of the agglutination colored band is shorter than a length of the expanded colored band in absence of the analytes.

2. The detection device as claimed in claim 1 , wherein the elongated carrier comprises a fibrous material.

3. The detection device as claimed in claim 1 , wherein the liquid sample moves toward the second end by capillary action.

4. The detection device as claimed in claim 1 to claim 3 , further comprising a housing having an inlet and forming an inner space, wherein the elongated carrier is disposed in the inner space with the first end of the elongated carrier facing toward the inlet, so that the liquid sample entering the inner space through the inlet is directed to the first end of the elongated carrier.

5. The detection device as claimed in claim 4 , further comprising a rod having a front end and an opposite rear end, wherein the rod is disposed in the inner space through an opening of the housing opposite to the inlet so that the rod is slidably disposed in the inner space and tightly fits to a sidewall of the housing with the rear end of the rod protruding out of the housing through the opening for applying a drawing process performed by a user.

6. The detection device as claimed in claim 5 , further comprising a needle body engagingly disposed at the inlet of the housing so as to direct the liquid sample into the inner space therethrough.

7. A detection kit, comprising:
an elongated carrier having a spiral segment with a plurality of loops along a central axis, and having a first end and an opposite second end, wherein the elongated carrier allows a liquid sample to move from the first end toward the second end and reach the spiral segment after the first end contacts with the liquid sample, wherein an agglutination colored band or an expanded colored band extending from the first end toward the second end of the elongated carrier is formed by a plurality of coloring elements in the liquid sample; and
a coating solution comprising a plurality of recognizing substances and a solvent, wherein the recognizing substances are fixed on the elongated carrier, the recognizing substances being able to bind to a plurality of analytes in the liquid sample so that the coloring elements form the agglutination colored band, wherein a length of the agglutination colored band is shorter than a length of the expanded colored band in absence of the analytes.

8. The detection kit as claimed in claim 7 , wherein the elongated carrier is made of fibrous material.

9. The detection kit as claimed in claim 7 , wherein the liquid sample moves toward the second end by capillary action.

10. The detection kit as claimed in claim 7 to claim 9 , further comprising a housing having an inlet and forming an inner space, wherein the elongated carrier is disposed in the inner space with the first end of the elongated carrier facing toward the inlet, so that the liquid sample entering the inner space through the inlet is directed to the first end of the elongated carrier.

11. The detection kit as claimed in claim 10 , further comprising a rod having a front end and an opposite rear end, wherein the rod is disposed in the inner space through an opening of the housing opposite to the inlet so that the rod is slidably disposed in the inner space and tightly fits to a sidewall of the housing with the rear end of the rod protruding out of the housing through the opening for applying a drawing process performed by a user.

12. The detection kit as claimed in claim 11 , further comprising a needle body engagingly disposed at the inlet of the housing so as to direct the liquid sample into the inner space therethrough.

13. A method for detection of a plurality of analytes in a liquid sample, comprising steps of:
providing an elongated carrier with a plurality of recognizing substances fixed thereon, wherein the elongated carrier has a first end and an opposite second end and a spiral segment with a plurality of loops along a central axis;
bringing the first end of the elongated carrier in contact with a liquid sample and allowing the liquid sample to move toward the second end along the elongated carrier and reach the spiral segment, therewith a colored band extending from the first end to the second end is formed on the elongated carrier by a plurality of coloring elements in the liquid sample; and
determining whether the liquid sample contains a plurality of analytes by distinguishing whether the colored band is an agglutination colored band or an expanded colored band based on the length of the colored band;
wherein when the liquid sample contains the analytes, the recognizing substances are able to bind to the analytes so that the coloring elements form the agglutination colored band, wherein a length of the agglutination colored band is shorter than a length of the expanded colored band in the absence of the analytes.

14. The method as claimed in claim 13 , wherein the elongated carrier comprises a fibrous material.

15. The method as claimed in claim 13 , wherein the liquid moves toward the second end by capillary action.

16. The method as claimed in claim 13 , wherein the detection method determines whether the analyte is contained in the liquid sample according to the number of the loops being colored by the liquid sample.

17. The method as claimed in claim 13 , wherein the detections method is performed by a detection unit as claimed in any one of claim 4 to claim 6 .

18. The method as claimed in any one of claim 13 to claim 17 , wherein the detection method is applied for identification of blood types.

19. The method as claimed in claims 18 , wherein the recognizing substances are antibody A or antibody B, the analytes are antigen A or antigen B, and the coloring elements are erythrocytes.

Assignees

• Nat Univ Tsing Hua

Inventors

• Cheng Chao-Min

Classifications

• G01N33/80 Primary

  involving blood groups or blood types {or red blood cells (white blood cells G01N33/56972)} · CPC title

• G01N33/538

  by sorbent column, particles or resin strip {, i.e. sorbent materials} · CPC title

• G01N33/54366

  Apparatus specially adapted for solid-phase testing · CPC title