Nucleic acid-controlled catalytic rnas for trigger-responsive regulation
US-2024425855-A1 · Dec 26, 2024 · US
US9683232B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9683232-B2 |
| Application number | US-37956409-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 25, 2009 |
| Priority date | Dec 10, 2007 |
| Publication date | Jun 20, 2017 |
| Grant date | Jun 20, 2017 |
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This relates to a method of preparing induced pluripotent stem cells, comprising a nuclear reprogramming step with a nuclear reprogramming factor in the presence of miRNA, wherein said miRNA has a property of providing a higher nuclear reprogramming efficiency in the presence of said miRNA than in the absence thereof.
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What is claimed is: 1. A method of preparing an induced pluripotent stem cell from a mammalian somatic cell, comprising nuclear reprogramming the mammalian somatic cell under culture conditions which allow for mammalian somatic cell reprogramming to pluripotency with at least one miRNA and an expression vector encoding at least one nuclear reprogramming factor, wherein the at least one miRNA increases efficiency of the nuclear reprogramming of the mammalian somatic cell compared t…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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