Yeast display of proteins in the periplasmic space
US-2024102202-A1 · Mar 28, 2024 · US
US9683028B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9683028-B2 |
| Application number | US-201414557171-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 1, 2014 |
| Priority date | Apr 17, 2000 |
| Publication date | Jun 20, 2017 |
| Grant date | Jun 20, 2017 |
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Methods useful in constructing libraries that collectively display and/or express members of diverse families of peptides, polypeptides or proteins and the libraries produced using those methods. Methods of screening those libraries and the peptides, polypeptides or proteins identified by such screens.
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We claim: 1. A vector comprising: (i) a DNA sequence encoding an antibody variable region linked to a fragment of a wild-type pIII anchor, which does not mediate infection of phage particles; and (ii) a wild-type gene III; wherein the nucleic acid sequence encoding the fragment of the wild-type pIII anchor in (i) differs from the portion of the wild-type gene III encoding the same fragment to an extent sufficient to reduce homologous recombination between the nucleic acid encoding…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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