Haploid induction compositions and methods for use therefor

Patent metadata

Field	Value
Publication number	US-9677082-B2
Application number	US-201414212504-A
Country	US
Kind code	B2
Filing date	Mar 14, 2014
Priority date	Mar 15, 2013
Publication date	Jun 13, 2017
Grant date	Jun 13, 2017

Abstract

Provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit haploid induction (HI); methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.

First claim

What is claimed is:

1. Method of creating a new haploid inducer maize plant with a silenced patatin-like phospholipase 2A, comprising transcribing a polynucleotide sequence capable of silencing the patatin-like phospholipase 2A in maize, wherein said polynucleotide sequence comprises a first sequence selected from the group consisting of:
a) a polynucleotide sequence comprising the nucleic acid sequence set forth in SEQ ID NO: 37 or the complement thereof,
b) a functional fragment comprising at least 22 contiguous bases of SEQ ID NO: 37 or the complement thereof, and
c) a polynucleotide sequence having at least 95% sequence identity as determined using the BLASTN alignment tool to the nucleic acid sequence set forth in SEQ ID NO: 37 or the complement thereof,
and a second sequence that is the complement of the first sequence, wherein the polynucleotide sequence expresses a double-stranded ribonucleotide sequence which silences the patatin-like phospholipase 2A when contacted with a maize plant and thus creates a new haploid inducer maize plant.

2. The method of claim 1 , wherein the contacting is achieved by transforming the plant with a polynucleotide sequence which when expressed produces a double-stranded ribonucleotide sequence capable of silencing the patatin-like phospholipase 2A.

Assignees

• Syngenta Participations Ag

Inventors

• CHINTAMANANI Satya
• Delzer Brent
• Nuccio Michael L
• Dietrich Robert Arthur
• Kadaru Suresh Babu
• Warner Todd Lee
• Bullock William Paul
• Kelliher Timothy

Classifications

• C12N15/8218 Primary

  Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS] · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• C12Y301/01002

  Arylesterase (3.1.1.2) · CPC title

• A01H1/08

  Methods for producing changes in chromosome number · CPC title

• A01H1/045

  using molecular markers · CPC title