Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system

Patent metadata

Field	Value
Publication number	US-9677042-B2
Application number	US-201113269351-A
Country	US
Kind code	B2
Filing date	Oct 7, 2011
Priority date	Oct 8, 2010
Publication date	Jun 13, 2017
Grant date	Jun 13, 2017

Abstract

Embodiments described herein generally relate to methods and systems for customizing protocols for use with a cell expansion system. Through a user interface, a user may create a custom task for loading, growing and/or harvesting cells. A custom task may comprise one or more steps, in which a user may add or omit steps, as desired. Data may be entered for settings associated with a custom task, in which embodiments provide for such data to be entered each time the custom task is performed. In another embodiment, the settings for a custom task may be configured, in which such settings may be stored and retrieved upon selection of the custom task. Customization and configuration of a custom task may occur using a diagram view of the cell expansion system, in which process settings are associated with graphical user interface elements.

First claim

What is claimed is:

1. A cell expansion system, comprising:
a bioreactor;
a user interface, wherein the user interface is operable to display data and receive user input;
a memory, wherein the memory contains a series of executable instructions; and
a processor in communication with the user interface and the memory, wherein the processor executes the series of executable instructions, and wherein the series of executable instructions cause the processor to:
receive, through the user interface, a first selection to create a first user-defined task for affecting a cell expansion in the bioreactor;
in response to receiving the first selection:
retrieve, from the memory, one or more settings for a first process of the first user-defined task;
provide, through the user interface, the one or more settings for the first process of the first user-defined task;
provide, through the user interface, a first graphical user interface element to add one or more processes to the first user-defined task;
receive a second selection to add the one or more processes to the first user-defined task;
in response to receiving the second selection:
retrieve, from the memory, one or more types of processes to add;
provide a selection window listing the one or more types of processes to add;
from the listing of the one or more types of processes to add, receive a third selection of a second process to add to the first user-defined task;
in response to receiving the third selection:
 retrieve, from the memory, one or more settings for the second process of the first user-defined task;
 provide, through the user interface, the one or more settings for the second process of the first user-defined task;

receive an indication to execute the first user-defined task; and
in response to receiving the indication to execute the first user-defined task:
execute the first process and the second process of the first user-defined task to affect the cell expansion in the bioreactor.

2. The cell expansion system as defined in claim 1 , wherein the one or more types of processes to add comprises one or more from the group consisting of: wash out lines, wash out lines through membrane, wash rapidly, harvest cells, add bolus, and user-defined.

3. The cell expansion system as defined in claim 1 , wherein the series of executable instructions cause the processor further to:
receive a fourth selection to configure the first process of the first user-defined task;
associate the first process of the first user-defined task with a first diagram view;
provide, through the user interface, the first diagram view;
retrieve a first setting of the one or more settings for the first process;
associate the first setting of the one or more settings for the first process with a second graphical user interface element;
provide, through the user interface, the second graphical user interface element in the first diagram view;
receive first data associated with the first setting of the one or more settings for the first process; and
store the first data associated with the first setting of the one or more settings for the first process.

4. The cell expansion system as defined in claim 1 , wherein the series of executable instructions cause the processor further to:
retrieve first data associated with at least a first setting of the one or more settings for the first process; and
provide, through the user interface, the first data associated with the at least the first setting of the one or more settings for the first process.

5. The cell expansion system as defined in claim 1 , wherein the series of executable instructions cause the processor, in response to receiving the second selection, further to:
provide first data associated with at least a first setting of the one or more settings for the second process; and
provide, through the user interface, the first data associated with the at least the first setting of the one or more settings for the second process.

6. The cell expansion system as defined in claim 3 , wherein the series of executable instructions cause the processor further to:
receive a fifth selection to configure the second process of the first user-defined task;
associate the second process of the first user-defined task with a second diagram view;
provide, through the user interface, the second diagram view;
retrieve a first setting of the one or more settings for the second process;
associate the first setting of the one or more settings for the second process with a third graphical user interface element;
provide, through the user interface, the third graphical user interface element in the second diagram view;
receive first data associated with the first setting of the one or more settings for the second process; and
store the first data associated with the first setting of the one or more settings for the second process.

7. The cell expansion system as defined in claim 3 , wherein the bioreactor comprises a hollow fiber membrane.

8. The cell expansion system as defined in claim 7 , wherein the hollow fiber membrane comprises an intracapillary space and an extracapillary space.

9. The cell expansion system as defined in claim 8 , wherein the providing the first diagram view comprises:
depicting the intracapillary space of the bioreactor in the first diagram view; and
depicting the extracapillary space of the bioreactor in the first diagram view.

10. The cell expansion system as defined in claim 3 , wherein the first setting of the one more settings for the first process comprises one or more from the group consisting of: intracapillary inlet, intracapillary inlet rate, intracapillary circulation rate, extracapillary inlet, extracapillary inlet rate, extracapillary circulation rate, rocker, and stop condition.

11. The cell expansion system as defined in claim 3 , wherein the series of executable instructions cause the processor further to:
determine whether the first setting of the one or more settings for the first process is associated with a numeric value; and
if the first setting of the one or more settings for the first process is associated with the numeric value, providing a data entry pad in the first diagram view to receive the numeric value.

12. The cell expansion system as defined in claim 11 , wherein the series of executable instructions cause the processor further to:
if the first setting of the one or more settings for the first process is not associated with the numeric value, determining if the first setting of the one or more settings for the first process is associated with a menu of selection options; and
if the first setting of the one or more settings for the first process is associated with the menu of selection options, providing the menu of selection options in the first diagram view.

Assignees

• Stanton Iv Edward Allan
• Antwiler Glen Delbert
• Howley Patrick J
• Kinzie Michael E
• Dodd Jon A
• Medina Casey V
• Terumo Bct Inc

Inventors

• Stanton Iv Edward Allan
• Antwiler Glen Delbert
• Howley Patrick J
• Kinzie Michael E
• Dodd Jon A
• Medina Casey V

Classifications

• C12M41/44 Primary

  of volume or liquid level · CPC title

• G06F8/38

  for implementing user interfaces · CPC title

• C12M23/44

  Multiple separable units; Modules · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M29/20

  Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02) · CPC title