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Heparin affinity tag and applications thereof
US9676816B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9676816-B2 |
| Application number | US-201414378230-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 21, 2014 |
| Priority date | Jan 21, 2014 |
| Publication date | Jun 13, 2017 |
| Grant date | Jun 13, 2017 |
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Abstract
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In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine.
First claim
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The invention claimed is: 1. An affinity tag for protein purification comprising: an amino acid sequence that comprises the sequence of SEQ ID NO:1. 2. The affinity tag of claim 1 , wherein the affinity tag binds heparin. 3. An affinity tag for protein purification comprising: an amino acid sequence that comprises the sequence of SEQ ID NO:2. 4. The affinity tag of claim 3 , wherein the affinity tag binds heparin.
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relating to the construction of the column · CPC title
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title
Packing or coating · CPC title
Phases chemically bonded to a substrate, e.g. to silica or to polymers · CPC title
containing a tag with affinity for a non-protein ligand · CPC title
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- What does patent US9676816B2 cover?
- In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag des…
- Who is the assignee on this patent?
- Univ Arkansas, Univ Arkansas
- What technology area does this patent fall under?
- Primary CPC classification C07K1/22. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 13 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).