Method for functionalizing surfaces for analyte detection

Patent metadata

Field	Value
Publication number	US-9671397-B2
Application number	US-201113880263-A
Country	US
Kind code	B2
Filing date	Oct 11, 2011
Priority date	Oct 18, 2010
Publication date	Jun 6, 2017
Grant date	Jun 6, 2017

Abstract

The invention relates to a device for detecting analytes, including a plastic substrate at least partially covered by bonding polymers attached to the substrate in a non-covalent manner, said bonding polymers comprising a polysaccharide backbone provided with aromatic groupings and carboxylic acid groupings.

First claim

The invention claimed is:

1. Device for detecting analytes, comprising a plastic substrate at least partly covered directly with bonding polymers fixed to the substrate non-covalently, said bonding polymers comprising a polysaccharide skeleton provided with:
aromatic groups of the form —X—CONH—Z, where X represents a linear or branched, substituted or unsubstituted alkyl chain, comprising 1 to 6 carbon atoms, and Z represents an aryl function;
carboxylic acid groups of the form —X—COOH, where X represents a linear or branched, substituted or unsubstituted alkyl chain, comprising 1 to 6 carbon atoms; and
reactive F groups selected from:
groups of the form —X—CONH—X′—N 3 ;
groups of the form —X—CONH—NH 2 ;
groups of formula (V):

groups of formula (V′):

where X and X′ each represent a substituted or unsubstituted alkyl chain comprising from 1 to 6 carbon atoms and Pep represents a peptide fragment.

2. Device according to claim 1 , in which the polysaccharide skeleton is a dextran skeleton.

3. Device according to claim 1 , in which:
X is CH 2 ; and/or
Z is —CH 2 -Ph or —CH 2 -Ph-paraOH.

4. Device according to claim 1 , in which the bonding polymers comprise:
from 0.4 to 0.8 aromatic groups per saccharide unit of the polysaccharide skeleton; and/or
from 0 to 0.8 reactive F groups, per saccharide unit of the polysaccharide skeleton; and/or
from 0.5 to 1.5 aromatic, carboxylic acid and reactive F groups in total, per saccharide unit of the polysaccharide skeleton.

5. Device according to claim 1 , in which the substrate is a substrate of polystyrene, polycarbonate, poly(methyl methacrylate) or polypropylene.

6. Device according to claim 1 , comprising capturing elements immobilized on the bonding polymers.

7. Device according to claim 6 , in which the capturing elements are selected from:
polypeptides;
saccharides, oligosaccharides and lipopolysaccharides;
viruses or virus fragments and cells.

8. Device according to claim 1 , comprising a plurality of detection zones.

9. Device according to claim 1 , in which the substrate is an opaque or transparent slide, a microtitre plate, a collection of beads, a culture plate, a strip or a stick.

10. Method of detecting chemical molecules, biological molecules, cells or living organisms comprising a step of contacting said chemical molecules, biological molecules, cells or living organisms with the device for detecting analytes according to claim 1 .

11. Bonding polymer that can be used in the device according to claim 1 comprising a polysaccharide skeleton provided with:
0.4 to 0.8 aromatic groups per saccharide unit of the form —X—CONH—Z, where X represents a linear or branched, substituted or unsubstituted alkyl chain, comprising 1 to 6 carbon atoms, and Z represents an aryl function;
carboxylic acid groups of the form —X—COOH, where X represents a linear or branched alkyl chain, comprising 1 to 6 carbon atoms; and
reactive F groups selected from:
groups of the form —X—CONH—X′—N 3 ;
groups of the form —X—CONH—NH 2 ;
groups of formula (V):

groups of formula (V′):

where X and X′ each represent a substituted or unsubstituted alkyl chain comprising from 1 to 6 carbon atoms and Pep represents a peptide fragment.

12. Bonding polymer according to claim 11 , in which the polysaccharide skeleton is a dextran skeleton.

13. Bonding polymer according to claim 11 , in which:
X and/or X′ is CH 2 ; and/or
X and/or X′ is (CH 2 ) 2 ; and/or
Z is —CH 2 -Ph or —CH 2 -Ph-paraOH.

14. Bonding polymer according to claim 11 , comprising:
from 0.4 to 0.6 aromatic groups per saccharide unit of the polysaccharide skeleton; and/or
from 0 to 0.8 reactive F groups per saccharide unit of the polysaccharide skeleton; and/or
from 0.5 to 1.5 aromatic, carboxylic acid and reactive F groups in total, per saccharide unit of the polysaccharide skeleton.

Assignees

• Melnyk Oleg
• Ebran Jean-Philippe Georges Bernard
• Dheur Julien Philippe
• Dendane Nabil
• Souplet Vianney
• Olivier Christophe
• Centre Nat Rech Scient
• Innobiochips
• Univ Lille Ii Droit & Sante
• Univ De Lille 1 Sciences Et Tech

Inventors

• Melnyk Oleg
• Ebran Jean-Philippe Georges Bernard
• Dheur Julien Philippe
• Dendane Nabil
• Souplet Vianney
• Olivier Christophe

Classifications

• G01N33/54393

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title

• G01N33/54353 Primary

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• C08B37/0021 Primary

  Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran · CPC title

• C09D105/02

  Dextran; Derivatives thereof · CPC title

• G01N2400/22

  Dextran · CPC title