Methods for reducing accumulation of lactate during culturing and method for producing polypeptide

Patent metadata

Field	Value
Publication number	US-9670519-B2
Application number	US-201214114525-A
Country	US
Kind code	B2
Filing date	Apr 27, 2012
Priority date	Apr 29, 2011
Publication date	Jun 6, 2017
Grant date	Jun 6, 2017

Abstract

The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.

First claim

We claim:

1. A method for reducing accumulation of lactate from 5% to 40% in a cell culture of Chinese Hamster Ovary (CHO cells) comprising:
a) growing said CHO cells in culture at 37±1° C. for 3-4 days in the presence of zinc salt at a concentration ranging from 0.2 mM to 0.4 mM;
b) reducing the temperature in the culture resulting from a) to 31±1° C. and further culturing said cells for 3-4 days, so as to reduce accumulation of lactate by 5% to 40% in the cell culture at the end of the culture period relative to a control which is not supplemental with zinc salt.

2. The method as claimed in claim 1 , wherein the zinc salt is zinc sulphate.

3. The method of claim 2 , wherein the zinc sulphate is zinc sulphate hepta hydrate salt.

4. The method as claimed in claim 1 , wherein the culturing is carried out in a system selected from the group consisting of fed batch culturing, batch culturing, shake flasks and bioreactor.

Assignees

• Srivastava Ruchika
• Hemdev Sneha Lakshmandas
• Bhatnagar Ankur
• Desan Saravanan
• Goel Anuj
• Iyer Harish
• Raja Vana
• Rao Lavanya
• Biocon Ltd

Inventors

• Srivastava Ruchika
• Hemdev Sneha Lakshmandas
• Bhatnagar Ankur
• Desan Saravanan
• Goel Anuj
• Iyer Harish
• Raja Vana
• Rao Lavanya

Classifications

• C07K16/2896

  against molecules with a "CD"-designation, not provided for elsewhere · CPC title

• C12N2500/10

  Metals; Metal chelators · CPC title

• C12N5/0018 Primary

  Culture media for cell or tissue culture (media for specific animal cell type C12N5/06) · CPC title

• C07K16/2863

  against receptors for growth factors, growth regulators · CPC title

• C07K16/2866

  against receptors for cytokines, lymphokines, interferons · CPC title