Isolated novel nucleic acid and protein molecules from soy and methods of using those molecules to generate transgenic plants with enhanced agronomic traits
US-9029636-B2 · May 12, 2015 · US
UDP-glycosyltransferase derived from ginseng and use thereof
US9670469B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9670469-B2 |
| Application number | US-201214431868-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 24, 2012 |
| Priority date | Sep 27, 2012 |
| Publication date | Jun 6, 2017 |
| Grant date | Jun 6, 2017 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention relates to a novel uridine diphosphate (UDP)-glycosyltransferase, and particularly to a novel UDP-glycosyltransferase derived from ginseng and use thereof, a method for preparing glycosylated ginsenoside by converting protopanaxadiol (PPD)-type ginsenoside using the UDP-glycosyltransferase, a composition for converting the PPD-type ginsenoside into glycosylated ginsenoside, comprising the UDP-glycosyltransferase, a transformant or a culture thereof as active ingredients, a method for enhancing the expression of the UDP-glycosyltransferase using MeJA (methyl jasmonate), and a composition for enhancing the expression of the UDP-glycosyltransferase, which comprises MeJA as an active ingredient.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for preparing a glycosylated ginsenoside by converting a protopanaxadiol (PPD)-type ginsenoside, comprising reacting the PPD-type ginsenoside with an isolated Uridine diphosphate (UDP)-glycosyltransferase protein of having the sequence of SEQ ID NO:1, a transformant introduced with an expression vector comprising a polynucleotide that encodes the protein having the sequence of SEQ ID NO:1, or a culture of the transformant comprising the protein having the sequence of SEQ ID NO:1 in the presence of UDP-sugar, wherein the UDP-glycosyltransferase protein has a sugar-transfer activity to the C-3 position of PPD-type ginsenoside and wherein the PPD-type ginsenoside is PPD or Compound K (C-K). 2. The method according to claim 1 , wherein the glycosylated ginsenoside is ginsenoside Rh2 or F2. 3. The method according to claim 1 , which further comprises reacting the glycosylated PPD-type ginsenoside with an isolated UDP-glycosyltransferase protein having the amino acid sequence of SEQ ID NO: 3, a transformant introduced with a vector comprising a polynucleotide that encodes the protein having the sequence of SEQ ID NO:3, or a culture of the transformant comprising the protein having the sequence of SEQ ID NO:3. 4. The method according to claim 3 , which comprises conversion of ginsenoside Rh2 into ginsenoside Rg3 or conversion of ginsenoside F2 into ginsenoside Rd by the isolated UDP-glycosyltransferase protein which is represented by the amino acid sequence of SEQ ID NO: 3.
Assignees
Inventors
Classifications
containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids · CPC title
Preparation of steroids · CPC title
- C12N9/1051Primary
Hexosyltransferases (2.4.1) · CPC title
having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin · CPC title
Glucuronosyltransferase (2.4.1.17) · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9670469B2 cover?
- The present invention relates to a novel uridine diphosphate (UDP)-glycosyltransferase, and particularly to a novel UDP-glycosyltransferase derived from ginseng and use thereof, a method for preparing glycosylated ginsenoside by converting protopanaxadiol (PPD)-type ginsenoside using the UDP-glycosyltransferase, a composition for converting the PPD-type ginsenoside into glycosylated ginsenosi…
- Who is the assignee on this patent?
- Korea Advanced Inst Sci & Tech, Intelligent Synthetic Biology Ct
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1051. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 06 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).