Bioreactor, reactor bag for same and stirrer for circulating contents of said bag

Patent metadata

Field	Value
Publication number	US-9670448-B2
Application number	US-201314435275-A
Country	US
Kind code	B2
Filing date	Aug 2, 2013
Priority date	Oct 18, 2012
Publication date	Jun 6, 2017
Grant date	Jun 6, 2017

Abstract

A bioreactor has a reactor bag ( 12 ) with a predominantly flexible bag wall ( 121 ) that has a rigid bearing area. A stirrer arrangement ( 14 ) is in the bearing area and includes rigid profile ring ( 16 ) made of a non-magnetic material and fixed in the bearing area. The stirrer arrangement ( 14 ) also includes a shaftless stirrer ( 18 ) rotatably engaged with the profile ring ( 16 ) and having parts that are permanently magnetic. An electric coil arrangement ( 20 ) is outside the reactor bag ( 12 ) and can produce a rotating magnetic field that interacts with the permanent-magnetic areas of the stirrer ( 18 ) to produce a torque that rotates the stirrer ( 18 ) and circulates the contents of the bag ( 12 ).

First claim

The invention claimed is:

1. A bioreactor, comprising
a reactor bag ( 12 ) having a predominantly flexible bag wall ( 121 ) with a rigidly formed bearing area in a segment of a height of the reactor bag ( 12 ),
a shaftless stirrer that is part of a rotor ( 18 ) that spans a cross-section of the bag ( 12 ), parts of the rotor ( 18 ) are permanently magnetic, the rotor ( 18 ) being housed in the bearing area inside of the bag,
a coil arrangement ( 20 ) positioned outside the reactor bag ( 12 ) and being operative to produce a rotating magnetic field that interacts with the permanent-magnetic parts of the rotor ( 18 ) to exert a torque on the rotor ( 18 ), and
a rigid profile ring ( 16 ) made of a non-magnetic material and having a guide rail ( 162 / 185 ), on which the rotor ( 18 ) is rotatably engaged, the profile ring ( 16 ) being arranged in the segment of the height of the reactor bag ( 12 ) along which the bearing area extends,
a magnetic field of the coil arrangement ( 20 ) interacting with at least one of the permanent magnet ( 186 ) parts arranged in a radially outer area of the rotor ( 18 ).

2. The bioreactor of claim 1 , wherein
the profile ring ( 16 ) penetrates the flexible bag wall ( 121 ) and is bonded to the flexible bag wall ( 121 ).

3. The bioreactor of claim 1 ,
wherein
the profile ring ( 16 ) has a guide groove ( 162 ) running around the profile ring ( 16 ), the guide groove ( 162 ) being open towards the interior of the bag, into which guide groove ( 162 ) at least one guide shoe ( 185 ) arranged on a radially outer part of the rotor ( 18 ) is introduced axially and radially.

4. The bioreactor of claim 3 ,
wherein
at no fewer than three positions on a circumference of the profile ring ( 16 ), radially oriented roller bearings ( 163 ) and/or axially oriented, opposed pairs of roller bearings ( 164 ) project with spring action into the guide groove ( 162 ).

5. The bioreactor of claim 3 ,
wherein
at no fewer than three positions on a circumference of the profile ring, radially oriented slide bearing springs and/or axially oriented, opposed pairs of slide bearing springs project into the guide groove.

6. The bioreactor of claim 3 ,
wherein
on an outer side of the profile ring ( 16 ), the guide groove ( 162 ) forms a projection with at least one axial mounting surface ( 167 ) on which an electric coil arrangement ( 20 ) designed as a separate unit or unit group is arranged, the coil arrangement ( 20 ) being capable of generating the magnetic field.

7. The bioreactor of claim 6 ,
wherein
the guide groove ( 162 ) on the outer side of the profile ring ( 16 ) is surrounded on three sides, by form-fitting connection, by an electric coil arrangement ( 20 ) designed as a separate unit or unit group, the coil arrangement ( 20 ) being capable of generating the magnetic field.

8. The bioreactor of claim 1 ,
wherein
the rotor ( 18 ) has a central body ( 181 ) and at least three spokes ( 184 ) extending radially from the central body ( 181 ).

9. The bioreactor of claim 8 ,
wherein
the spokes ( 184 ) of the rotor ( 18 ) are connected to one another by a ring-shaped rim ( 185 ).

10. The bioreactor of claim 9 ,
wherein
the rim ( 185 ) has a plurality of permanent magnets ( 186 ) distributed around its circumference.

11. A reactor bag for a bioreactor ( 10 ), comprising: a predominantly flexible bag wall ( 121 ) with a rigid bearing area in a segment of a height of the reactor bag ( 12 ), and
a rigid profile ring ( 16 ) made of a non-magnetic material arranged in the bearing area in the segment of the height of the reactor bag ( 12 ) along which the bearing area extends, the profile ring ( 16 ) defining a guide rail ( 162 / 185 ).

12. The reactor bag of claim 11 ,
wherein
the profile ring ( 16 ) penetrates the flexible bag wall ( 121 ) and is bonded to the flexible bag wall ( 121 ).

13. The reactor bag of claim 11 , further comprising:
a stirrer arranged in the interior of the bag and defining a shaftless rotor ( 18 ) spanning a cross-section of the bag and housed rotatably in an axial and radial manner on the profile ring ( 16 ) by means of the guide rail ( 162 / 185 ).

14. A stirrer to circulate the contents of a bioreactor ( 10 ) comprising:
a plurality of paddle-shaped circulation elements ( 182 ), a holder ( 183 ) at least partly surrounding the circulation elements ( 182 ) and fastened to the circulation elements ( 182 ),
at least three spokes ( 184 ) extending radially out from the holder ( 183 ), and
at least one rim ring ( 185 ) engaged with radially outer ends of the spokes ( 184 ), the at least one rim ring ( 185 ) having at least one permanent magnet ( 186 ) arranged in the at least one rim ring ( 185 ) and configured for engagement with an outer guide rail ( 162 ).

15. The stirrer of claim 14 ,
wherein
the spokes ( 184 ) of the rotor ( 18 ) are connected to one another by the rim ring ( 185 ), the at least one permanent magnet ( 186 ) defining a plurality of permanent magnets ( 186 ) distributed around the rim ring ( 185 ).

Assignees

• Sartorius Stedim Biotech Gmbh

Inventors

• Zeuch Stefan
• Regen Thomas
• Greller Gerhard
• Reif Oscar-Werner

Classifications

• C12M27/02 Primary

  Stirrer or mobile mixing elements · CPC title

• B01F15/0085

  Operations & Transport · mapped topic

• C12M23/14 Primary

  Bags · CPC title

• C12M23/28

  disposable or single use · CPC title

• B01F13/0827

  Operations & Transport · mapped topic