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Culture medium composition and method of culturing cell or tissue using thereof
US9664671B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9664671-B2 |
| Application number | US-201313949310-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 24, 2013 |
| Priority date | Jul 24, 2012 |
| Publication date | May 30, 2017 |
| Grant date | May 30, 2017 |
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- Title
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Abstract
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The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like.
First claim
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The invention claimed is: 1. A composition comprising (a) a culture medium, (b) deacylated gellan gum or a salt thereof, and (c) adherent cells, wherein the adherent cells are pluripotent stem cells in the form of spheres, wherein the deacylated gellan gum or salt thereof is present at a concentration which allows the spheres to be cultured in suspension standing culture, and wherein the composition has a viscosity of not more than 8 mPa-s at 37° C. 2. The composition according to claim 1 , wherein the composition has a concentration of the deacylated gellan gum or a salt thereof of 0.01-0.05% (weight/volume). 3. The composition according to claim 1 , which further comprises a polysaccharide other than deacylated gellan gum or a salt thereof. 4. The composition according to claim 1 , which comprises a metal ion. 5. The composition according to claim 4 , wherein the metal ion is a calcium ion. 6. The composition according to claim 4 , wherein deacylated gellan gum or a salt thereof is assembled or forms a three-dimensional network via the metal ion. 7. The composition according to claim 1 , wherein the pluripotent stem cells are proliferating pluripotent stem cells.
Assignees
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Classifications
for testing toxicity · CPC title
Calcium; Ca chelators; Calcitonin · CPC title
Protein-free medium · CPC title
Microcarriers · CPC title
Tumour cells; Cancer cells · CPC title
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- What does patent US9664671B2 cover?
- The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, …
- Who is the assignee on this patent?
- Nissan Chemical Ind Ltd, Univ Kyoto
- What technology area does this patent fall under?
- Primary CPC classification G01N33/5011. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue May 30 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).