Polyacrylamide electrophoresis gels with protection against oxygen exposure

Patent metadata

Field	Value
Publication number	US-9664646-B2
Application number	US-201314085472-A
Country	US
Kind code	B2
Filing date	Nov 20, 2013
Priority date	Nov 20, 2012
Publication date	May 30, 2017
Grant date	May 30, 2017

Abstract

The detrimental effect of oxygen exposure on the formation of polyacrylamide electrophoresis gels in oxygen-permeable plastic cassettes is reduced or eliminated either by the use of an oxygen barrier material over the surfaces of the plastic walls of the cassette through which oxygen would otherwise pass into the cassette cavity, or by the incorporation of an oxygen scavenger in the plastic from which the cassette walls are made, or both.

First claim

What is claimed is:

1. A combination of a polyacrylamide gel and a plastic cassette in which said polyacrylamide gel resides, the plastic cassette comprising:
cassette walls enclosing a gel cavity and comprising a matrix plastic that is permeable to oxygen, and
a material comprising an oxygen barrier layer covering walls of said cassette such that the oxygen barrier layer is in contact with said polyacrylamide gel, wherein the walls of the cassette are formed by injection molding and the oxygen barrier layer is applied to the walls of the cassette by in-mold decoration,
wherein the material inhibits oxygen access to the polyacrylamide gel through the cassette walls.

2. The combination of claim 1 wherein said oxygen barrier layer is a layer of a member selected from the group consisting of polyvinylidene chloride and low-density polyethylene.

3. The combination of claim 1 wherein said oxygen barrier layer has an oxygen permeability at 25° C. and 100% RH of 5×10 −10 [cm 3 cm]/[cm 2 s (cm Hg)] or less.

4. The combination of claim 1 wherein the material further comprises an oxygen scavenger embedded within said matrix.

5. The combination of claim 4 wherein said oxygen scavenger is a member selected from the group consisting of a hydroxyl-functionalized polybutadiene oligomer, a crystalline polycondensation product of a meta-xylylene diamine and adipic acid, and a nylon 6 nanocomposite.

6. The combination of claim 1 wherein said matrix plastic is a member selected from the group consisting of styrene-acrylonitrile polymer, polyethylene terephthalate, glycol-modified polyethylene terephthalate, polyethylene naphthalate, and polycarbonate.

7. A method for casting a polyacrylamide gel from acrylamide monomer in a plastic cassette having cassette walls comprising a matrix plastic that is permeable to oxygen while inhibiting exposure of said acrylamide monomer to oxygen, said method comprising:
(a) providing said cassette, wherein said cassette is made by covering walls of said cassette that will be in contact with said polyacrylamide gel with an oxygen barrier layer, wherein the walls of the cassette are formed by injection molding and the oxygen barrier layer is applied to the walls of the cassette by in-mold decoration; and
(b) casting said polyacrylamide gel from an acrylamide monomer solution in said plastic cassette.

8. The method of claim 7 wherein said oxygen barrier layer is a layer of a member selected from the group consisting of polyvinylidene chloride and low-density polyethylene.

9. The method of claim 7 wherein said oxygen barrier layer has an oxygen permeability at 25° C. and 100% RH of 5×10 −10 [cm 3 cm]/[cm 2 s (cm Hg)] or less.

10. The method of claim 7 wherein said matrix plastic further comprises an oxygen scavenger incorporated therein.

11. The method of claim 10 wherein said oxygen scavenger is a member selected from the group consisting of a hydroxyl-functionalized polybutadiene oligomer, a crystalline polycondensation product of a meta-xylylene diamine and adipic acid, and a nylon 6 nanocomposite.

12. The method of claim 7 wherein said matrix plastic is a member selected from the group consisting of styrene-acrylonitrile polymer, polyethylene terephthalate, glycol-modified polyethylene terephthalate, polyethylene naphthalate, and polycarbonate.

Assignees

• Bio Rad Laboratories Inc

Inventors

• Mckee Jason
• Panattoni Cory

Classifications

• G01N27/44756 Primary

  Apparatus specially adapted therefor · CPC title

• G01N27/44747 Primary

  Composition of gel or of carrier mixture · CPC title

• G01N27/453

  Cells therefor · CPC title