Electrochemical detoxification method of wood-based hydrolysate for producing biochemicals or biofuels

Patent metadata

Field	Value
Publication number	US-9657318-B2
Application number	US-201514874040-A
Country	US
Kind code	B2
Filing date	Oct 2, 2015
Priority date	Apr 4, 2013
Publication date	May 23, 2017
Grant date	May 23, 2017

Abstract

Provided is a method for preparing a lignocellulosic biomass hydrolysate having toxicity reduced or removed and being used for fermentation by microorganisms, comprising the steps of: pretreating, hydrolyzing, and using an electrochemical detoxification method.

First claim

The invention claimed is:

1. A method for preparing a lignocellulosic biomass hydrolysate, the lignocellulosic biomass hydrolysate having toxicity reduced or removed and being used for fermentation by microorganisms,
wherein the method comprises:
pretreating a lignocellulosic biomass; and
hydrolyzing the pretreated biomass, and
wherein the method further comprises electrochemically treating the pretreated biomass or the hydrolyzed biomass to generate radicals by oxidizing toxic compounds, thereby reducing or removing the toxicity of the hydrolysate, and
wherein the resulting lignocellulosic biomass hydrolysate comprises a phenolic compound reduced by 50 wt % or more as compared to before electrochemical treatment, and comprising a furanic compound reduced by 90 wt % or more as compared to before electrochemical treatment.

2. The method for preparing a lignocellulosic biomass hydrolysate with toxicity reduced or removed according to claim 1 , wherein the phenolic compound is one or more selected from a group consisting of ferulic acid, coumaric acid, benzoic acid, syringic acid, vanillic acid, vanillin, 4-hydroxybenzoic acid, 4-hydroxybenzaldehyde and syringaldehyde.

3. The method for preparing a lignocellulosic biomass hydrolysate with toxicity reduced or removed according to claim 1 , wherein the furanic compound is one or more selected from a group consisting of furfural and 5-hydroxymethylfurfural (5-HMF).

4. The method for preparing a lignocellulosic biomass hydrolysate with toxicity reduced or removed according to claim 1 , wherein the electrochemical treatment comprises flowing an electric current.

5. The method for preparing a lignocellulosic biomass hydrolysate with toxicity reduced or removed according to claim 1 , wherein the electrochemical treatment comprises applying a voltage of 0.01-50 V.

6. The method for preparing a lignocellulosic biomass hydrolysate with toxicity reduced or removed according to claim 1 , which further comprises, before the electrochemical treatment, determining an optimum voltage for oxidizing a toxic compound contained in the hydrolysate, and the electrochemical treatment comprises applying the determined voltage.

7. A method for preparing a biochemical or a biofuel, comprising,
preparing a lignocellulosic biomass hydrolysate according to claim 1 , and
fermenting the lignocellulosic biomass hydrolysate.

8. The method for preparing a biochemical or a biofuel according to claim 7 , wherein the fermentation comprises adding microorganisms to the hydrolysate and culturing the added microorganisms.

9. The method for preparing a biochemical or a biofuel according to claim 8 , wherein the microorganism is one or more selected from the group consisting of yeast, Lactic acid bacteria, Clostridium, E. coli and Bacillus.

10. The method for preparing a biochemical or a biofuel according to claim 7 , wherein the biochemical is one or more of a fatty acid, a diol, a diene and an organic acid.

11. The method for preparing a biochemical or a biofuel according to claim 7 , wherein the biofuel is ethanol or butanol.

Assignees

• Korea Inst Sci & Tech

Inventors

• Um Youngsoon
• Kim Yunje
• Suh Dongjin
• Lee Kyung Min
• Ha Jeong Myeong

Classifications

• Y02E50/343

  Cross-Sectional Technologies · mapped topic

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P7/10 Primary

  substrate containing cellulosic material · CPC title

• Y02E50/16

  Cross-Sectional Technologies · mapped topic

• C12P2201/00

  Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis · CPC title