Decreasing nitrite content in tobacco via expression of a nitrite reductase

Patent metadata

Field	Value
Publication number	US-9657303-B2
Application number	US-201514601186-A
Country	US
Kind code	B2
Filing date	Jan 20, 2015
Priority date	Aug 15, 2007
Publication date	May 23, 2017
Grant date	May 23, 2017

Abstract

The present invention relates in one aspect to a method for producing a transgenic plant, comprising introducing into an unmodified plant an exogenous gene encoding a nitrite reductase, wherein expression of the nitrite reductase encoded by the exogenous gene reduces nitrite content in the transgenic plant relative to the unmodified plant. Also provided are transgenic plants and plant cells comprising an exogenous gene encoding a nitrite reductase, as well as associated uses, chimaeric genes and plant transformation vectors.

First claim

The invention claimed is:

1. A transgenic Nicotiana tabacum plant comprising:
an exogenous gene encoding a nitrite reductase having at least 95% amino acid sequence identity to the polypeptide as defined in SEQ ID NO:3, wherein the gene encodes a protein having nitrite reductase activity and a plastid transit peptide, wherein nitrite content in the transgenic plant is reduced compared to an unmodified plant.

2. The transgenic plant according to claim 1 , wherein the exogenous gene is from a plant of the genus Arabidopsis.

3. The transgenic Nicotiana tabacum plant according to claim 2 , wherein the exogenous gene comprises the sequence as defined in SEQ ID NO:2.

4. The transgenic Nicotiana tabacum plant according to claim 1 , wherein the exogenous gene encoding the nitrite reductase is associated with a promoter sequence capable of directing constitutive expression of the nitrite reductase in the transgenic plant.

5. The transgenic Nicotiana tabacum plant according to claim 4 , wherein the promoter sequence is a constitutive promoter from Carnation Etched Ring Virus.

6. The transgenic Nicotiana tabacum plant according to claim 1 , wherein the plant is a primary transgenic plant generated by introduction of the exogenous gene into a wild type Nicotiana tabacum plant.

7. The transgenic Nicotiana tabacum plant according to claim 6 , wherein the primary transgenic plant contains a single copy of the exogenous gene.

8. The transgenic Nicotiana tabacum plant according to claim 1 , wherein the plant is a secondary or subsequent generation transgenic plant derived from propagation of a primary transgenic plant, the primary transgenic plant being generated by introduction of the exogenous gene into a wild type plant.

9. The transgenic Nicotiana tabacum plant according to claim 8 , wherein the second or subsequent generation transgenic plant is homozygous for the exogenous gene encoding the nitrite reductase.

10. The transgenic Nicotiana tabacum plant according to claim 1 , wherein the nitrite reductase encoded by the exogenous gene is expressed in leaves of the transgenic plant.

11. The transgenic Nicotiana tabacum plant according to claim 1 , wherein the nitrite content of the transgenic plant is at least 10% lower compared to a wild type Nicotiana tabacum plant.

Assignees

• British American Tobacco Investments Ltd

Inventors

• Davenport Susie
• Maunders Martin

Classifications

• C12Y107/02001

  Nitrite reductase (NO-forming) (1.7.2.1) · CPC title

• C12N9/0044

  acting on other nitrogen compounds as donors (1.7) · CPC title

• C12N5/14

  Plant cells · CPC title

• A01H1/06

  Processes for producing mutations, e.g. treatment with chemicals or with radiation (specific mutations prepared by genetic engineering on plant cell or plant tissues C12N15/00 {; process for producing transgenic plants C12N15/82}) · CPC title

• C12N15/8243 Primary

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title