Bacteriophage and antibacterial composition comprising the same

Patent metadata

Field	Value
Publication number	US-9657277-B2
Application number	US-201414770429-A
Country	US
Kind code	B2
Filing date	Feb 24, 2014
Priority date	Feb 27, 2013
Publication date	May 23, 2017
Grant date	May 23, 2017

Abstract

Provided is a novel bacteriophage ΦCJ19 (KCCM11361P). In addition, the present invention relates to an antibacterial composition including the bacteriophage ΦCJ19 (KCCM11361P) as an active ingredient. Further, the present invention is a method of preventing and/or treating infectious diseases by enterotoxigenic Escherichia coli in animals except for humans using the bacteriophage ΦCJ19 (KCCM11361P) or the antibacterial composition containing the bacteriophage ΦCJ19 (KCCM11361P) as an active ingredient.

First claim

The invention claimed is:

1. A method of preparing an animal feed composition, the method comprising:
providing a bacteriophage composition comprising a bacteriophage having the nucleic acid sequence of SEQ ID No: 1; and
mixing the composition with an animal feed base to provide the animal feed composition such that the bacteriophage is in an amount of 1×10 6 to 5×10 12 PFU/ml with reference to the total amount of the animal feed composition.

2. The method of claim 1 , further comprising:
mixing, with the animal feed base, one or more additives selected from the group consisting of an amino acid, a vitamin, an enzyme, a probiotic, a flavoring agent, a non-protein nitrogen compound, a silicate, a buffer, a coloring agent, an extractant and an oligosaccharide.

3. A method of feeding, the method comprising:
preparing the animal feed composition according to the method of claim 1 ; and
feeding, to an animal, the animal feed composition as a feed.

4. A method of preparing a drinking water composition, the method comprising:
providing a bacteriophage composition comprising a bacteriophage having the nucleic acid sequence of SEQ ID No: 1; and
mixing the composition with drinking water to provide the drinking water composition such that the bacteriophage is in an amount of 1×10 6 to 5×10 12 PFU/ml with reference to the total amount of the drinking water composition.

5. The method of claim 4 , further comprising:
mixing, with the animal feed base, one or more additives selected from the group consisting of an amino acid, a vitamin, an enzyme, a probiotic, a flavoring agent, a non-protein nitrogen compound, a silicate, a buffer, a coloring agent, an extractant and an oligosaccharide.

6. A method of feeding, the method comprising:
preparing the drinking water composition according to the method of claim 4 ; and
feeding, to an animal, the drinking water composition as drinking water.

7. A method of treating an infectious disease caused by ETEC, the method comprising:
administering a bacteriophage composition to a subject in need of such treatment, wherein the bacteriophage composition comprises a pharmaceutically acceptable carrier and a bacteriophage having the nucleic acid sequence of SEQ ID No: 1 in an amount of 1×10 6 to 5×10 12 PFU/ml with reference to the total amount of the bacteriophage composition.

8. A method of claim 7 , wherein the infectious disease is colibacillosis.

9. A method of retarding progress of an infectious disease caused by ETEC, the method comprising:
feeding an animal feed composition to an animal, wherein the animal feed composition comprising an animal feed base and a bacteriophage having the nucleic acid sequence of SEQ ID No: 1 in an amount of 1×10 6 to 5×10 12 PFU/ml with reference to the total amount of the animal feed composition.

10. A method of claim 9 , wherein the infectious disease is colibacillosis.

Assignees

• Cj Cheiljedang Corp

Inventors

• Shin Eun Mi
• Bae Gi Duk
• Kim Jae Won

Classifications

• C12N2795/10231

  Uses of virus other than therapeutic or vaccine, e.g. disinfectant · CPC title

• A23K50/30

  for swines · CPC title

• A23K20/195

  Antibiotics · CPC title

• A61K35/76

  Viruses; Subviral particles; Bacteriophages · CPC title

• A61P31/04

  Antibacterial agents · CPC title